Goat Anti-Mouse Anti-Rabbit IgG/IgM H&L (HRP polymer) (ab2891)

Submit a review Q&A (11)References (21)

Key features and details

  • Goat Anti-Mouse Anti-Rabbit IgG/IgM H&L (HRP polymer)
  • Conjugation: HRP polymer
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, IHC-Fr

Overview

  • Product name

    Goat Anti-Mouse Anti-Rabbit IgG/IgM H&L (HRP polymer)
    See all IgG + IgM secondary antibodies

  • Host species

    Goat

  • Target species

    Mouse

  • Specificity

    This antibody reacts with heavy and light chains on mouse and rabbit IgG. It reacts with all mouse and rabbit IgG subclasses and with mouse and rabbit IgM.

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details

  • Conjugation

    HRP polymer

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituents: 0.04% Proclin 300, 0.5% Proclin 950, 99% Water
  • Concentration information loading...

  • Conjugation notes

    HRP conjugated to a polymer backbone, which is collectively conjugated to the antibody. The effect is multiple HRP molecules linked to one single antibody.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    This new and innovative HRP-polymerization technology provides a significant increase in staining sensitivity when compared to other conventional HRP-conjugated secondary antibodies. Avidin-biotin blocking procedures are not necessary when using this product. The overall staining procedure uses one less reagent (streptavidin peroxidase) and rinsing step, eliminates avidin-biotin blocking procedures and may reduce overall staining time.

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution. This product is provided in a pre-diluted, ready-to-use format. Apply for 20 - 30 minutes.
Notes
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution. This product is provided in a pre-diluted, ready-to-use format. Apply for 20 - 30 minutes.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (21)

Publishing research using ab2891? Please let us know so that we can cite the reference in this datasheet.

ab2891 has been referenced in 21 publications.

  • Xin Z  et al. The immune landscape of human thymic epithelial tumors. Nat Commun 13:5463 (2022). PubMed: 36115836
  • Hashemibeni B  et al. Investigation and Comparison of the Effect of TGF-ß3, kartogenin and Avocado/Soybean Unsaponifiables on the In-vitro and In-vivo Chondrogenesis of Human Adipose-Derived Stem Cells on Fibrin Scaffold. Iran J Pharm Res 20:368-380 (2021). PubMed: 34903995
  • Zhao Z  et al. Gut Microbiota Was Involved in the Process of Liver Injury During Intra-Abdominal Hypertension. Front Physiol 12:790182 (2021). PubMed: 34955896
  • Feng C  et al. Tumor-Suppressive MicroRNA-216b Binds to TPX2, Activating the p53 Signaling in Human Cutaneous Squamous Cell Carcinoma. Mol Ther Nucleic Acids 20:186-195 (2020). PubMed: 32169806
  • Hadziselimovic F  et al. Abnormal histology in testis from prepubertal boys with monorchidism. Basic Clin Androl 30:11 (2020). PubMed: 32782807
View all Publications for this product

Customer reviews and Q&As

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1-10 of 11 Abreviews or Q&A

Question

Inquiry: I am thinking of ordering your product #ab2891 Goat anti-mouse HRP Polymer. I will be using it on frozen and paraffin sections of various tissue types. I noticed it is a 6 ml bottle. What is your recommended dilution range for both frozen and paraffin sections and does it come in larger quantities? Thank you. Donna Suresch

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Answer

Thank you very much for contacting us with your question.
Ab2891 is a prediluted antibody, and it is intended to be used without further dilution, on both frozen and paraffin sections. We don't have larger quantities at this time, but if you need 60 mL or more (10+ bottles), we can arrange a bulk order for you. Please contact sales@abcam.com if this is the case.
I hope this information will be useful, but please let me know if you have further questions or need anything else and I'll be happy to help.

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Question

What is the expiry date for ab2891?

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Answer

Thank you for contacting us.

The expiry date would be 1 year i.e. untill 31st July 2013.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

number of HRP molecules per polymer?
% increase in signal intensity compared to regular HRP-secondary Ab?

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Answer

Thank you for contacting us.

Ihave have received another replyfrom the supplying lab, with a few more details for question 2) especially:

#1) Detection polymer composition is proprietary and that detail cannot be disclosed.

#2) We have not run our polymer detection side-by-side with a non-polymer HRP detection to allow us to give a quantitative answer. We can say that the polymer detection affords a significant increase in sensitivity that will allow users to increase primary antibody titers and/or decrease incubation times.

I hope this information is of some more help.Please do not hesitate to contact us if you need any more advice or information.

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Question

number of HRP molecules per polymer?
% increase in signal intensity compared to regular HRP-secondary Ab?

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Answer

Thank you for contacting us.
I have heard back from the supplying lab:

Unfortunately, the answer to the first question (number of HRP molecules per polymer) is proprietary.

As for the secondquestion (% increase in signal intensity compared to regular HRP-secondary antibody): The answer would be subject tomany varying conditions, such asfixation, signal in tissue, section of tissue, HIER, etc. We do not have a general answer that applies to these circumstances in this case.

I am very sorry that I do not have more detailed information which I can give you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Can you tell me if this product contains the Fc fragment?

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Answer

Thank you for your enquiry. Unfortunately, we are not sure if this product contains the Fc fragment.

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Question

I have already purchased the secondary Ab ab2891 - now I realise it is pre-diluted for IHC. If I want to use it for Western Blotting what dilution can I use it at?

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Answer

Thank you for your enquiry. Unfortunately since it has not been previously tested in Western blot and since we do not know the concentration of the secondary antibody (this information is considered confidential by the antibody originator), I can only make a guess at the dilution you would need to use. You may wish to try a few different dilution, I might try a range from 1/10 to 1/100 to begin with, and then see what the staining looks like and adjust the dilutions accordingly. Please do let us know how you make out with this antibody in Western blot, as we would love to know whether it will work in this application and what dilution can be used. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

Can you tell me the concetration of this antibody? Can you tell me what the protein carrier and preservative are? If all of this information is proprietary, can you at least tell me if the protein carrier or preservative are likely to cross-react with galectin-3?

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Answer

Thank you for your enquiry and your patience. This antibody is a polymer detection for mouse and rabbit antibodies on human tissue. It will cross-react with virtually all monoclonals and rabbits antibodies. Specific cross-reactivity with a particular protein has not been observed. As I mentioned on the telephone, the concentration, protein carrier and preservative are proprietary. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

Dear Technical Team, A customer wants to know the concentration of ab2891? Would you have a rough estimate? Thank you for your time and help.

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Answer

Thank you for your enquiry. Further to correspondence with the source of this antibody unfortunately I have been unable to determine a precise concentration. They are unable to disclose the antibody concentrations of their detection systems. I can however tell you that this product is provided in a pre-diluted, ready-to-use format. I am sorry that I cannot be of more assistance.

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Question

I need to know the concentration of the ab2891 prediluted antibody and how much I can further dilute it in order to use it for ELISA?

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Answer

I am terribly sorry for the delay in finding the answer to your question regarding the concentration of ab2891. Unfortunately the originator of this antibody cannot disclose this information to us as it is proprietary and must remain confidential. I apologise for the inconvenience,

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Question

This product is pre-diluted - what is the dilution factor? How much should be used?

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Answer

This product is provided in a pre-diluted, ready-to-use format for immunostaining procedures. There is no need to dilute further this secondary antibody. Please refer to our IHC protocols for more information, and if you have any further questions, please do let us know.

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1-10 of 11 Abreviews or Q&A

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