Goat Anti-Pig IgG H&L (Alkaline Phosphatase) (ab6916)
Key features and details
- Goat Anti-Pig IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Fr
Related conjugates and formulations
Overview
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Product name
Goat Anti-Pig IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Pig -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details -
Immunogen
Full length native Swine IgG (purified).
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using pig IgG coupled to agarose beads -
Conjugation notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/200 - 1/1000.
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IHC-P |
Use at an assay dependent dilution.
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WB |
Use at an assay dependent dilution.
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ELISA |
1/2000 - 1/10000.
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Immunomicroscopy |
Use at an assay dependent dilution.
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Dot blot |
Use at an assay dependent dilution.
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IHC-Fr |
Use at an assay dependent dilution.
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Notes |
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ICC/IF
1/200 - 1/1000. |
IHC-P
Use at an assay dependent dilution. |
WB
Use at an assay dependent dilution. |
ELISA
1/2000 - 1/10000. |
Immunomicroscopy
Use at an assay dependent dilution. |
Dot blot
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab6916 has been referenced in 4 publications.
- Premkumar L et al. The receptor binding domain of the viral spike protein is an immunodominant and highly specific target of antibodies in SARS-CoV-2 patients. Sci Immunol 5:N/A (2020). PubMed: 32527802
- Premkumar L et al. The RBD Of The Spike Protein Of SARS-Group Coronaviruses Is A Highly Specific Target Of SARS-CoV-2 Antibodies But Not Other Pathogenic Human and Animal Coronavirus Antibodies. medRxiv N/A:N/A (2020). PubMed: 32511572
- Fiebig U et al. Antibody Cross-Reactivity between Porcine Cytomegalovirus (PCMV) and Human Herpesvirus-6 (HHV-6). Viruses 9:N/A (2017). WB . PubMed: 29143761
- Rampton M et al. Antibody responses to Sarcoptes scabiei apolipoprotein in a porcine model: relevance to immunodiagnosis of recent infection. PLoS One 8:e65354 (2013). WB . PubMed: 23762351