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Goat Anti-Rabbit IgG Fc (HRP) (ab97200)

Submit a review Q&A (5)References (63)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG Fc (HRP) (ab97200)
  • Western blot - Goat Anti-Rabbit IgG Fc (HRP) (ab97200)

Key features and details

  • Goat Anti-Rabbit IgG Fc (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

Overview

  • Product name

    Goat Anti-Rabbit IgG Fc (HRP)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rabbit

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG.

  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

  • Conjugation notes

    Molar enzyme/ antibody protein ratio is 4:1

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
ELISA
1/10000 - 1/100000. (Primary ELISA)
WB
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000)
Notes
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
ELISA
1/10000 - 1/100000. (Primary ELISA)
WB
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000)

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG Fc (HRP) (ab97200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG Fc (HRP) (ab97200)

    IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97200, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat Anti-Rabbit IgG Fc (HRP) (ab97200)
    Western blot - Goat Anti-Rabbit IgG Fc (HRP) (ab97200)

    ab97200 was used at dilution 1/5000 with the primary antibody ab90045 in WB. See the review on ab90045.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (63)

Publishing research using ab97200? Please let us know so that we can cite the reference in this datasheet.

ab97200 has been referenced in 63 publications.

  • Liskova V  et al. Camptothecin, triptolide, and apoptosis inducer kit have differential effects on mitochondria in colorectal carcinoma cells. FEBS Open Bio 12:913-924 (2022). PubMed: 35318813
  • Cai G  et al. SIRT4 functions as a tumor suppressor during prostate cancer by inducing apoptosis and inhibiting glutamine metabolism. Sci Rep 12:12208 (2022). PubMed: 35842463
  • Cabral SE  et al. Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in Xenopus oocytes. iScience 25:104811 (2022). PubMed: 35982794
  • Benitez A  et al. Nerve Growth Factor: A Dual Activator of Noradrenergic and Cholinergic Systems of the Rat Ovary. Front Endocrinol (Lausanne) 12:636600 (2021). PubMed: 33716987
  • Huang Q  et al. 0.5-Gy X-ray irradiation induces reorganization of cytoskeleton and differentiation of osteoblasts. Mol Med Rep 23:N/A (2021). PubMed: 33760136
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Question

I will use anti-osteopontin rabbi policlonal ABCAM ab91655 for Western blott purpose. I need a secondary antibody

HRP labelled goat anti-rabbit as suggested by the site.

Should I use ab97200 or ab97069 ? Or maybe another one?

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Answer


I would actually recommend an alternative secondary antibody ab97051, which is part of our AbExcel series of secondary antibodies. The link for ab97051 is given below:
https://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-hl-hrp-ab97051.html
Information about our AbExcel antibodies and why they are preferable for western blotting:
https://www.abcam.com/index.html?pageconfig=resource&rid=13534&source=pagetrap
We also provide a number of Western blot reagents and kits including our Optiblot ECL, ECL Max and ELC Ultra Detect kits which our enhanced chemiluminescent substrates specially developed for film imaging. These Optiblot ECL Detect Kits produce strong signal with very low background for high sensitivity. More information about our ECL kits and our Optiblot range may be found at the links below:
Optiblot ECL Detect Kit (23pg-187ng): https://www.abcam.com/Optiblot-ECL-Detect-Kit-23pg-187ng-ab133406.html
Optiblot ECL Max Detect Kit (4.6pg-4.7ng): https://www.abcam.com/Optiblot-ECL-Max-Detect-Kit-46pg-47ng-ab133408.html
Optiblot ECL Ultra Detect Kit (1.2pg-2ng): https://www.abcam.com/Optiblot-ECL-Ultra-Detect-Kit-12pg-2ng-ab133409.html
Our entire Optiblot range: https://www.abcam.com/index.html?pageconfig=resource&rid=14709

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Question

ab7778und Proben vom Schwein

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Answer

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unserem Wissen nach wurde ab7778 bisher noch nicht inSchwein getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zurzeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab7778 abdecken, und gegen eine erneute Bestellung von uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

1.) Bestätigen Sie mir bitte, dass Sie ab7778 inSchwein testen möchten.

2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

4.) Testen Sie den Antikörper in Schwein.

5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
https://www.abcam.com/abreviews

6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können ein anderes Produkt zu dem gleichen Preis wie ab7778 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab7778 bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/collaborationdiscount

Die Datenblätter für die zwei passenden sekundären Antikörper, die wir am Telefon besprochen haben,können über diese Links eingesehen werden:

https://www.abcam.com/Goat-Rabbit-IgG-Fc-DyLight-488-ab96983.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-Fc-DyLight-488-ab96983.html).

https://www.abcam.com/Goat-Rabbit-IgG-Fc-HRP-ab97200.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-Fc-HRP-ab97200.html).

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

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Question

Dear All.



Please let us have alternate product information on the items of Life Technologies as below:



Catalogue of competitor-656120

Catalogue of competitor-610120



Sincere regards,

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Answer

Thank youf or your inquiry.

We have many goat anti rabbit IgG HRP conjugated antibodies:

Please use the links below to review the datasheets of three of them:

ab97051

https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-ab97051.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-ab97051.html).

ab97080

https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-preadsorbed-ab97080.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-preadsorbed-ab97080.html).

ab97200

https://www.abcam.com/Goat-Rabbit-IgG-Fc-HRP-ab97200.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-Fc-HRP-ab97200.html).

Unfortunately, we do not have any rabbit anti mouse IgG1 HRP conjugated antibodies in our catalog. But do have anti mouse IgG1 antibodies from other host animals like goat and rat:

ab97240

https://www.abcam.com/Goat-Mouse-IgG1-HRP-ab97240.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG1-HRP-ab97240.html).

ab20043

https://www.abcam.com/Goat-Mouse-IgG-Fc-HRP-ab20043.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG-Fc-HRP-ab20043.html).

ab99603

https://www.abcam.com/Rat-monoclonal-SB77e-Mouse-IgG1-HL-HRP-preadsorbed-ab99603.html (or use the following: https://www.abcam.com/Rat-monoclonal-SB77e-Mouse-IgG1-HL-HRP-preadsorbed-ab99603.html).

I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

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Question

Hi Abcam,

I tried contacting the abcam distributer here in Australia (Sapphire bioscience), but the representative was unable to answer my questions.

I would like to purchase one secondary antibody against primary abcam antibodies I already have.

I would like to use the antibodies in a dot blot assay, using the ECL detection method.

There are three possible secondary antibodies that seem to me like they will work for two of my primaries:

Primary: ab8898 and ab9051
Secondary: ab97064, ab97095 and ab97200

Which secondary should I choose that will be best for both of my primaries, using ECL detection in a Dot Blot assay?

Thank you in advance for your assistance.

Read More
Answer

Thank you for contacting us.

The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not tested them specifically for this application and thus cannot guarantee that it would work.

Therefore, I would like to recommend one of the following secondaries which have been tested in dot blot:

ab6721: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-ab6721.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-ab6721.html).

ab6802: https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-ab6802.html (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-ab6802.html).

ab6795: https://www.abcam.com/Sheep-Rabbit-IgG-HL-HRP-ab6795.html (or use the following: https://www.abcam.com/Sheep-Rabbit-IgG-HL-HRP-ab6795.html).

They are raised in goat, donkey and sheep and apart from this are equally well suited for your experimental setup. If you need a decision guiding you could for example choose the one with the most references: this would be ab6721 with 52 publications mentioning the use of this antibody.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (https://www.abcam.com/Abpromise).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

antibodies class specific againts

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Answer

We have been informed by the source of the secondary antibodies ab97195 and ab97200 that : By immunoelectrophoresis and ELISA, ab97195 (anti-Rabbit IgM - HRP) reacts specifically with rabbit IgM. This antibody may cross react with IgM from other species.   By immunoelectrophoresis and ELISA,  ab97200 (anti-Rabbit IgG - HRP) reacts specifically with rabbit IgG-Fc. This antibody may cross react with IgG from other species.

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