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Goat Anti-Rabbit IgG H&L (ab2892)

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Key features and details

  • Goat Anti-Rabbit IgG H&L
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, IHC-Fr, ICC

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy3.5 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 12nm Gold 6nm HRP HRP polymer IRDye® 800CW PE Texas Red ® TRITC Unconjugated

Overview

  • Product name

    Goat Anti-Rabbit IgG H&L
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rabbit

  • Specificity

    This antibody cocktail reacts with heavy and light chains on rabbit IgG and mouse IgG (reacts with all IgG subtypes).

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, ICCmore details

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituents: 0.04% Proclin 300, 0.5% Proclin 950, 99% Water
  • Concentration information loading...

  • Conjugation notes

    HRP or AP conjugated to a polymer backbone, which is collectively conjugated to the antibody. The end result is two antibody subsets: 1) Multiple HRP molecules linked to goat anti-rabbit antibodies. 2) Multiple AP molecules linked to goat anti-mouse antibodies.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    This product is specially designed for a rapid double stain procedure. The overall staining procedure can be done in 4 steps and in less than two hours. Avidin-biotin blocking procedures are not necessary when using this product. This new and innovative AP and HRP polymerization technology provides a significant increase in staining sensitivity when compared to other conventional AP or HRP conjugated secondary antibodies.

    This product is provided in a pre-diluted, ready-to-use format. Procedure (adapt in-house protocols to accommodate): 1) A primary antibody cocktail with a rabbit monoclonal/polyclonal and a mouse monoclonal/polyclonal antibody must be applied for the standard duration. Rinse in TBS. (DO NOT USE PBS). 2) Apply ab2892 for 20 - 30 minutes then rinse in TBS (DO NOT USE PBS). 3) Chromogen 1: Develop AP using suitable chromogen. Rinse in TBS. 4) Chromogen 2: Develop HRP using suitable chromogen. Rinse in TBS. Equally suited to both manual and automated immunostaining proceedures. This product has not been tested in any application requiring further dilution of the antibody and therefore it is not recommended for use in such applications.

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/1.
IHC-Fr
1/1.
ICC
1/1.
Notes
IHC-P
1/1.
IHC-Fr
1/1.
ICC
1/1.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab2892? Please let us know so that we can cite the reference in this datasheet.

ab2892 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Question

How many pH of TBS recommed to used in ab2892 procudure? Procedure (adapt in-house protocols to accommodate): 1) A primary antibody cocktail with a rabbit monoclonal/polyclonal and a mouse monoclonal/polyclonal antibody must be applied for the standard duration. Rinse in TBS. (DO NOT USE PBS). 2) Apply ab2892 for 20 - 30 minutes then rinse in TBS (DO NOT USE PBS). 3) Chromogen 1: Develop AP using suitable chromogen. Rinse in TBS. 4) Chromogen 2: Develop HRP using suitable chromogen. Rinse in TBS.

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Answer

Thank you for your enquiry. The TBS buffer than we recommend is a general TBS buffer (pH7.6). Please find below the ingredients for the buffer. TBS 10x (concentrated TBS) - 24.23 g Trizma HCl - 80.06 g NaCl - Mix in 800 ml ultra pure water. - pH to 7.6 with pure HCl. - Top up to 1 L. TBS - For 1 L: 100 ml of TBS 10x + 900 ml ultra pure water Further more information on buffers and stock solutions, please see the pdf file link below. https://www.abcam.com/ps/pdf/protocols/Buffers%20and%20stock%20solutions.pdf If there is anything else that I can help you with, please do not hesitate to contact me.

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Question

I have a scientist enquiry regarding ab2892. She wants to know if this kit can be used for a single stain as well as a double stain? If not, would Abcam have a mouse and rabbit single staining kit? Thank you for your time and help.

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Answer

Thank you for your enquiry. Yes, this can be used to perform single staining. However, I would recommend that your customer uses an HRP or AP conjugated secondary antibody together with a suitable chromogen as this would be a more simplistic approach. For example: Goat anti-rabbit IgG antibody (ab7090). However, I appreciate that we do not sell an AP conjugated anti-mouse secondary and if this was the approach it may be necessary to use this kit for detection purposes.

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Question

Thanks for reading this message. I am interested in knowing the reason on why you suggested to use TBS instead of PBS in the application note for catalog# ab2892 (mouse + rabbit double staining secondary antibody). Do you typically get better results with TBS as compared to PBS? If we are using a phosphor antibody, would you recommend using TBS for both the primary and the secondary antibody stain?

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Answer

Thank you for your enquiry and your interest in our products. We normally suggest TBS instead of PBS for IHC staining. For details on IHC and antigen retrieval, please click on the Resources section on the top panel of the Abcam homepage, you might find some useful information there. As a general rule, for phosphor antibody we would suggest blocking the non-specific binding sites with 1%BSA rather than with milk. We hope this will help.

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Question

Customer would like to know: using ab2892, how are the mouse and rabbit differentiated? Are they different colors?

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Answer

The originator of ab2892 was able to provide me with the following information: mouse (AP)will appear red and rabbit (HRP) will appear brown.

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Question

Hi, I would like to do a double IHC stain on human tissue on paraffin tissue sections using anti-BMRF1 (monoclonal ab raised in mouse) and anti-Gastrin (polyclonal ab raised in rabbit). Is there any kit, or any protocol you are familiar with to do double Immunohistochemical staining? Thank you,

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Answer

Sorry but we do not have a protocol for double staining although we do plan to create a general double staining protocol in the next few months. Please keep an eye on the protocol section on the Abcam website for details.

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