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    products/secondary-antibodies/goat-rabbit-igg-hl-alexa-fluor-680-preadsorbed-ab186696.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed (ab186696)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

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Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed (ab186696)

    Key features and details

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed
    • Conjugation: Alexa Fluor® 680. Ex: 679nm, Em: 702nm
    • Host species: Goat
    • Suitable for: WB

    Conjugates logo Related conjugates and formulations

    Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy3.5 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 12nm Gold 6nm HRP HRP polymer IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated

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    Overview

    • Product name

      Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed
      See all IgG secondary antibodies
    • Host species

      Goat
    • Target species

      Rabbit
    • Specificity

      By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, horse, human, mouse, pig, and rat IgG was detected. This antibody may cross react with IgG from other species.

       

    • Tested applications

      Suitable for: WBmore details
    • Minimal
      cross-reactivity


      Chicken, Cow, Horse, Human, Mouse, Pig, Rat
      To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
      more details
    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Conjugation

      Alexa Fluor® 680. Ex: 679nm, Em: 702nm

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Antiserum was cross adsorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
    • Clonality

      Polyclonal
    • General notes

      We batch test Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed, ab186696 in fluorescent WB. Although we don’t batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed, ab186696 in these applications.

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Rabbit
      • IgG
      • Fluorophore
      • Alexa Fluor® 680
      • Secondary antibodies
      • anti-Rabbit
      • IgG
      • Fluorophore
      • Alexa Fluor® 680

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab186696 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/10000.
    Notes
    WB
    1/10000.

    Images

    • Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed (ab186696)
      Western blot - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed (ab186696)
      Anti-beta Tubulin antibody - Loading Control (ab6046) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed (ab186696) at 1/10000 dilution

      Observed band size: 50 kDa why is the actual band size different from the predicted?



      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6046 overnight at 4°C. Antibody binding was detected using ab186696 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab186696? Please let us know so that we can cite the reference in this datasheet.

    ab186696 has been referenced in 2 publications.

    • Bocca C  et al. The Metabolomic Bioenergetic Signature of Opa1-Disrupted Mouse Embryonic Fibroblasts Highlights Aspartate Deficiency. Sci Rep 8:11528 (2018). PubMed: 30068998
    • Tang S  et al. GOLPH3: a novel biomarker that correlates with poor survival and resistance to chemotherapy in breast cancer. Oncotarget 8:105155-105169 (2017). PubMed: 29285241

    Customer reviews and Q&As

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