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Goat Anti-Rabbit IgG H&L (APC) preadsorbed (ab130805)

Submit a review Q&A (1)References (13)

Key features and details

  • Goat Anti-Rabbit IgG H&L (APC) preadsorbed
  • Conjugation: APC. Ex: 645nm, Em: 660nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: WB, ICC/IF, Flow Cyt, ELISA, IHC-Fr, IHC-P

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase beta-galactosidase Biotin Cy2 ® Cy3 ® Cy3.5 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 12nm Gold 6nm HRP HRP polymer IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated

Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (APC) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rabbit

  • Specificity

    Reacts with the heavy and light chains of Rabbit IgG, and with the light chains of Rabbit IgM. Goat Anti-Rabbit IgG H&L (Allophycocyanin) preadsorbed shows minimal cross reactivity with Human and Mouse immunoglobulins.

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, ELISA, IHC-Fr, IHC-Pmore details

  • Minimal
    cross-reactivity


    Human, Mouse more details

  • Immunogen

    Full length protein corresponding to Rabbit IgG.

  • Conjugation

    APC. Ex: 645nm, Em: 660nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.3
    Preservative: 0.1% Sodium azide
    Constituents: PBS, Sucrose
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab130805 was purified by affinity chromatography on pooled IgG covalently linked to agarose. ab130805 has been cross absorbed with purified Human paraproteins, pooled Mouse sera, and pooled Mouse plasmacytoma/hybridoma proteins.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab130805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.
ICC/IF
Use 0.1µg for 106 cells.
Flow Cyt
Use at an assay dependent concentration.

use at ≤ 0.1 µg/106 cells
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration.
ICC/IF
Use 0.1µg for 106 cells.
Flow Cyt
Use at an assay dependent concentration.

use at ≤ 0.1 µg/106 cells
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (13)

Publishing research using ab130805? Please let us know so that we can cite the reference in this datasheet.

ab130805 has been referenced in 13 publications.

  • Castañeyra-Ruiz L  et al. AQP4 labels a subpopulation of white matter-dependent glial radial cells affected by pediatric hydrocephalus, and its expression increased in glial microvesicles released to the cerebrospinal fluid in obstructive hydrocephalus. Acta Neuropathol Commun 10:41 (2022). PubMed: 35346374
  • Shi S  et al. Wnt3a knockdown promotes collagen type II expression in rat chondrocytes. Exp Ther Med 24:526 (2022). PubMed: 35837029
  • Wang Y  et al. Neutrophils recruited to immunization sites initiating vaccine-induced antibody responses by locally expressing BAFF. iScience 25:104453 (2022). PubMed: 35874922
  • Zhang H  et al. Transplantation of GMP-grade human iPSC-derived retinal pigment epithelial cells in rodent model: the first pre-clinical study for safety and efficacy in China. Ann Transl Med 9:245 (2021). PubMed: 33708872
  • Shi Y  et al. Low molecular weight heparin (nadroparin) improves placental permeability in rats with gestational diabetes mellitus via reduction of tight junction factors. Mol Med Rep 21:623-630 (2020). PubMed: 31974593
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Customer kindly contacted us to inquire about the immunogen sequence for ab56215.

Read More
Answer

Thank you for contacting us today regarding secondary antibodies.

I have made a short list of compatible antibodies for use withab56215 Anti-SLC4A4 antibody.These products are pre-absorbed to ensure minimum cross reactivity. Each product is conjugated a different fluorochome so that you may chose the one which best fits your experiments. Please let me know if you have any questions.

AMCA:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-AMCA-ab123435.html

PE:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-PE-pre-adsorbed-ab72465.html

DyLight 594:https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-594-pre-adsorbed-ab98500.html

Texas Red:https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-Pre-Adsorbed-ab7088.html

APC:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-Allophycocyanin-pre-adsorbed-ab130805.html

If testing live cells we also recommend cell viability controls such as 7-AAD orPropidium Iodide.Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. We do carry PI and offer a 7-AADCell-Mediated Cytotoxicity Assay Kit.

Propidium Iodide: https://www.abcam.com/Propidium-Iodide-ab14083.html

7-AAD Kit:https://www.abcam.com/7-AADCFSE-Cytotoxicity-Assay-Kit-ab133073.html



In reviewing the datasheet for this product I did note that it has not been tested in Flow Cytometry. As such we cannot guarantee ab56212 in that application. However by participating in our AbTrial program, you can now use our products in an untested application or species without financial risk.



Simply follow these easy steps below to take part in our AbTrial Program:

1. Reply to this e-mail to let me know that you would like to proceed and test this product in Flow Cytometry.

2. I will email you aninactivepersonal discount code for the value of the product.

3. Purchase and test the product at regular price.

4. https://www.abcam.com/abreviews with you results noting your AbTrial discount code in the additional notes section.

5. Once the Abreview is submitted, the discount code will becomeactive.

6. Apply your discount code on your next order to receive that value off.

More information including the terms and conditions about this program may be found at: https://www.abcam.com/AbTrial

I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.

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