Goat Anti-Rabbit IgG H&L (APC) preadsorbed (ab130805)
Key features and details
- Goat Anti-Rabbit IgG H&L (APC) preadsorbed
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ICC/IF, Flow Cyt, ELISA, IHC-Fr, IHC-P
Related conjugates and formulations
Overview
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Product name
Goat Anti-Rabbit IgG H&L (APC) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
Reacts with the heavy and light chains of Rabbit IgG, and with the light chains of Rabbit IgM. Goat Anti-Rabbit IgG H&L (Allophycocyanin) preadsorbed shows minimal cross reactivity with Human and Mouse immunoglobulins.
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Tested applications
Suitable for: WB, ICC/IF, Flow Cyt, ELISA, IHC-Fr, IHC-Pmore details -
Minimal
cross-reactivity
Human, Mouse more details -
Immunogen
Full length protein corresponding to Rabbit IgG.
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Conjugation
APC. Ex: 645nm, Em: 660nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.3
Preservative: 0.1% Sodium azide
Constituents: PBS, Sucrose -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab130805 was purified by affinity chromatography on pooled IgG covalently linked to agarose. ab130805 has been cross absorbed with purified Human paraproteins, pooled Mouse sera, and pooled Mouse plasmacytoma/hybridoma proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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ICC/IF |
Use 0.1µg for 106 cells.
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Flow Cyt |
Use at an assay dependent concentration.
use at ≤ 0.1 µg/106 cells |
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. |
ICC/IF
Use 0.1µg for 106 cells. |
Flow Cyt
Use at an assay dependent concentration. use at ≤ 0.1 µg/106 cells |
ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab130805 has been referenced in 10 publications.
- Zhang H et al. Transplantation of GMP-grade human iPSC-derived retinal pigment epithelial cells in rodent model: the first pre-clinical study for safety and efficacy in China. Ann Transl Med 9:245 (2021). PubMed: 33708872
- Shi Y et al. Low molecular weight heparin (nadroparin) improves placental permeability in rats with gestational diabetes mellitus via reduction of tight junction factors. Mol Med Rep 21:623-630 (2020). PubMed: 31974593
- Osorio EY et al. In-situ proliferation contributes to the accumulation of myeloid cells in the spleen during progressive experimental visceral leishmaniasis. PLoS One 15:e0242337 (2020). PubMed: 33180876
- Momozane T et al. Efficient Differentiation of Mouse Induced Pluripotent Stem Cells into Alveolar Epithelium Type II with a BRD4 Inhibitor. Stem Cells Int 2019:1271682 (2019). PubMed: 31949433
- Lin W et al. Lgr5-overexpressing mesenchymal stem cells augment fracture healing through regulation of Wnt/ERK signaling pathways and mitochondrial dynamics. FASEB J N/A:fj201900082RR (2019). PubMed: 30991839
- Fukui E et al. Adipose Tissue-Derived Stem Cells Have the Ability to Differentiate into Alveolar Epithelial Cells and Ameliorate Lung Injury Caused by Elastase-Induced Emphysema in Mice. Stem Cells Int 2019:5179172 (2019). PubMed: 31281377
- Tao D et al. Accumulation of advanced glycation end products potentiate human retinal capillary endothelial cells mediated diabetic retinopathy. Mol Med Rep 20:3719-3727 (2019). PubMed: 31432194
- Zhang N et al. Expression of arginase I and inducible nitric oxide synthase in the peripheral blood and lymph nodes of HIV-positive patients. Mol Med Rep 13:731-43 (2016). Flow Cyt ; Human . PubMed: 26647762
- Marion-Poll L et al. Fluorescence-activated sorting of fixed nuclei: a general method for studying nuclei from specific cell populations that preserves post-translational modifications. Eur J Neurosci 39:1234-44 (2014). Flow Cyt . PubMed: 24713002
- Kim JJ et al. Targeted inhibition of serotonin type 7 (5-HT7) receptor function modulates immune responses and reduces the severity of intestinal inflammation. J Immunol 190:4795-804 (2013). PubMed: 23554310