Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
Key features and details
- Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
- Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISA
Related conjugates and formulations
Overview
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Product name
Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISAmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
Immunogen
Full length native Rabbit IgG (purified).
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Conjugation
Cy3 ®. Ex: 552nm, Em: 565nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. -
Conjugation notes
Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 9.9 moles Cy3 per mole of Goat IgG -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labelled without unwanted cross reaction.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
1/1000 - 1/5000.
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IHC-Fr | (1) |
Use at an assay dependent dilution.
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IHC-P |
1/100. PubMed: 17045908
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ICC |
Use at an assay dependent dilution.
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Flow Cyt |
1/500 - 1/2500.
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ELISA |
1/10000 - 1/50000.
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Notes |
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ICC/IF
1/1000 - 1/5000. |
IHC-Fr
Use at an assay dependent dilution. |
IHC-P
1/100. PubMed: 17045908 |
ICC
Use at an assay dependent dilution. |
Flow Cyt
1/500 - 1/2500. |
ELISA
1/10000 - 1/50000. |
Images
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Histone modifications associated with NOR-1 and NOR-2 loci in root tip cycling cells of Q.robur.
IFF detection of H3K9ac (A), H3K4me3 (B), H3K9me1 (C) and H3K27me2 (D). Green signals correspond to immunofluorescence of histone antibodies and red signals represent 18S rDNA FISH signals. Letter “n” marks the nucleoli. Scale bar is 5 µm.
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Photomicrographs showing immunostaining of (Panel A) ABCG2 (red), (Panel B) Bmi-1 (green), (Panel C) C/EBPδ (red), (Panel D) PCNA (red), (Panel E) CK18 (green), and (Panel F) cleaved caspase-3 (red) in HOK (human oral keratinocytes) cells cultured for five days without subsequent storage (control).
Photomicrographs are representative of four independent samples.
Cell nuclei were counterstained with DAPI (blue).
Original magnification: 200x.
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Examples of the different antibody stainings for functional analyses.
Left Panel: Ki-67 staining (Cy-3, red), HEK-293 transfected with NKX2-5-YFP (green).
Right Panel: Cyclin B staining (Cy-3, red), PANC-1 transfected with PRKCZ-YFP (green).
Original magnifications are indicated in the images.
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Lane M: 3 µL Molecular ladder.
Lane 1: Rabbit IgG whole molecule (p/n 011-0102).
Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105).
Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103).
Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107).
Lane 5: Normal Rabbit Serum (p/n B309).
All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.
Primary Antibody: ab6939 used at 1/1000 for 60 min at RT.
Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody used at 1/40000 in blocking buffer for 30 min at RT.
Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.
Rabbit F(c) migrates slightly higher.
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Lane 1: Rabbit IgG.
Load: 50 ng per lane.
Secondary antibody: ab6939 at 1/1000 for 60 min at RT. Blocked for 30 min at RT.
Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Rabbit IgG.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (227)
ab6939 has been referenced in 227 publications.
- Woike D et al. The Shank/ProSAP N-Terminal (SPN) Domain of Shank3 Regulates Targeting to Postsynaptic Sites and Postsynaptic Signaling. Mol Neurobiol 61:693-706 (2024). PubMed: 37656313
- Li XJ et al. Allicin ameliorates sepsis-induced acute kidney injury through Nrf2/HO-1 signaling pathway. J Nat Med 78:53-67 (2024). PubMed: 37668824
- You Y et al. Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy. Nat Biomed Eng N/A:N/A (2023). PubMed: 36635419
- Shafique S et al. Gestational exposure to triphenyl phosphate induces epigenetic modifications in C57Bl/6 fetal liver. Birth Defects Res 115:338-347 (2023). PubMed: 36369707
- Chen L et al. JMJD3 Is Required for Acute Pancreatitis and Pancreatitis-Associated Lung Injury. J Immunol 210:180-190 (2023). PubMed: 36458991