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    products/secondary-antibodies/goat-rabbit-igg-hl-dylight-550-ab96884.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rabbit IgG H&L (DyLight® 550) (ab96884)

  • Datasheet
Submit a review Q&A (2)References (15)

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Goat Anti-Rabbit IgG H&L (DyLight® 550) (ab96884)

    Key features and details

    • Goat Anti-Rabbit IgG H&L (DyLight® 550)
    • Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: ICC/IF, IHC-P, Flow Cyt

    Conjugates logo Related conjugates and formulations

    Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy3.5 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 12nm Gold 6nm HRP HRP polymer IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated

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    Overview

    • Product name

      Goat Anti-Rabbit IgG H&L (DyLight® 550)
      See all IgG secondary antibodies
    • Host species

      Goat
    • Target species

      Rabbit
    • Specificity

      By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
    • Tested applications

      Suitable for: ICC/IF, IHC-P, Flow Cytmore details
    • Immunogen

      Other Immunogen Type corresponding to Rabbit IgG.

    • Conjugation

      DyLight® 550. Ex: 562nm, Em: 576nm

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • General notes

      DyLight® 550 replaces DyLight® 549.
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Rabbit
      • IgG
      • Fluorophore
      • DyLight® 550

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab96884 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    1/50 - 1/500.
    IHC-P
    1/50 - 1/500.

    DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.

    Flow Cyt
    1/50 - 1/200.
    Notes
    ICC/IF
    1/50 - 1/500.
    IHC-P
    1/50 - 1/500.

    DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.

    Flow Cyt
    1/50 - 1/200.

    Images

    • Goat Anti-Rabbit IgG H&L (DyLight® 550) (ab96884)
      Goat Anti-Rabbit IgG H&L (DyLight® 550) (ab96884)
      Emission spectra of DyLight® fluorochromes available in our catalog.
      Line colors represent the approximate visible colors of the wavelength maxima.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (15)

    Publishing research using ab96884? Please let us know so that we can cite the reference in this datasheet.

    ab96884 has been referenced in 15 publications.

    • Iwamoto K  et al. Autologous transplantation of multilayered fibroblast sheets prevents postoperative pancreatic fistula by regulating fibrosis and angiogenesis. Am J Transl Res 13:1257-1268 (2021). PubMed: 33841654
    • Chen CF  et al. Characterization of Osteogenesis and Chondrogenesis of Human Decellularized Allogeneic Bone with Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Wharton's Jelly. Int J Mol Sci 22:N/A (2021). PubMed: 34445692
    • Cuello F  et al. Impairment of the ER/mitochondria compartment in human cardiomyocytes with PLN p.Arg14del mutation. EMBO Mol Med 13:e13074 (2021). PubMed: 33998164
    • Nagase T  et al. Allogeneic fibroblast sheets accelerate cutaneous wound healing equivalent to autologous fibroblast sheets in mice. Am J Transl Res 12:2652-2663 (2020). PubMed: 32655797
    • Li HY  et al. Matrix sieving-enforced retrograde transcytosis regulates tissue accumulation of C-reactive protein. Cardiovasc Res 115:440-452 (2019). PubMed: 29992240
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    We have finally moved into our new lab space. We will hopefully begin to
    start doing some experiments and testing those samples you had given us and
    then placing some orders. We have received an email about a new Igf2 antibody
    that should work in rat and mouse. Would you be able to send us a sample
    (and its control peptide) to also test by immunohistochemistry and western?
    I would really appreciate it!

    Read More

    Abcam community

    Verified customer

    Asked on Jan 26 2012

    Answer

    My colleague forwarded your message about testing an Abcam IGF2 antibody to me.

    As he mentioned, if western blotting and IHC are listed as applications on the datasheet, and rat and mouse are listed, then the antibody will be guaranteed for those applications and species and a replacement or credit or refund is available in the event it fails. If not, it is not guaranteed.

    For untested applications or species, we do not have samples, but we offer the testing discount he mentioned.

    The procedure is as follows: I first issue a discount code to you. Then, your lab purchases the antibody, tests it, and sends us your results in the form of a review (www.abcam.com/abreviews has some information about this). Once we have your review, your testing discount code becomes active, regardless of whether the results are positive or negative.

    The discount is a credit that can be applied to a subsequent order, good for one free primary antibody of your choice. I will be happy to issue you a code before you purchase. I just need the antibody catalogue number, and I will send you a code, which you include in the notes section of the review form.

    For some examples of customer reviews, please see the datasheet for anti-IGF2 ab9574:

    Click here (or use the following: https://www.abcam.com/IGF2-antibody-ab9574.html).

    Regarding a control peptide, this is available for some antibodies, but not all. If so, it will be a separate item for purchase.

    I look forward to your reply.

    Read More

    Abcam Scientific Support

    Answered on Jan 26 2012

    Question

    I am seeing high background in HMEK ICC staining.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 12 2011

    Answer

    I've been following up on the issues that we spoke about earlier in regards to the Anti-E Cadherin (phospho S838 + S840) antibody. I have also received your corresponence and thank you for taking the time to send that to me. I'll try to answer all the questions that you have raised and try to get these antibodies working for you. "1. Could primary Ab be nonspecifically attached to plastic in 96 well plate, causing high background?" Although there is a possibility your findings that "Minimal difference between groups 1 and 2 – cells with or without primary antibodies, possibly because of high background intensity" leads me to believe that background problems are a result of the secondary antibody.   "2. How to reduce the intensity of background: extra wash after introducing  primary Ab, going beyond ABcam recommended dilution rate for the secondary Ab?" I would definitly recommend decreasing the concentration of the secondary antibody as well as blocking in 5% BSA for 1 hour at room temp.  "3. Should we try to use higher concentration of primary Ab to possibly engage more e-cadherin sites for secondary Ab to differentiate from the background? Could that also increase the background?" This may help I would recommend doing this if you still have target recognition issues but only after you have been able to reduce your background staining. "4. Do we really need to conduct the cell fixation, and could that cause the cell loss in the experiment?" We spoke of the need to perform fixation to preserve protein morpholoy and expose epitopes. It is very possible that changing the fixation to acetone or methanol will greatly help you experiments. "5. Could cell permealization add to the amount of reacting epitopes?" I've found that the epitope site recognized by this antibody is intracelluar. Permeablization, whether by a detergents such as NP-40 or Triton-X 100 is neccessary if the fixative does not permeablize (eg , formalin, pfa), the acetone fixation that we spoke of will permeablize the cells so a seperate step will no be needed. I've included a pdf detailing ICC fixation and permeablization steps for you (see attached). Here is the SwissProt page where I found that information: http://www.uniprot.org/uniprot/P12830 "6. Conducting the experiment on  glass to prevent possible non-specific secondary Ab  attachment  to plastic causing high background? Could that also contribute to the higher cell loss after fixation? Could that possibly improve the resolution?" Although glass may help , I would definitely work with changing other aspect of the protocol first. Here is a link to our standard ICC protocol as I have not found a product specific one: https://www.abcam.com/index.html?pageconfig=resource&rid=12129 I hope that these suggestions help. Please feel free to contact us with any other questions that you have.   

    Read More

    Abcam Scientific Support

    Answered on Dec 12 2011

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