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    products/secondary-antibodies/goat-rabbit-igg-hl-dylight-650-preadsorbed-ab96902.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96902)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (9)

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Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96902)

    Key features and details

    • Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed
    • Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: IHC-P, ICC/IF, Flow Cyt

    Conjugates logo Related conjugates and formulations

    Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy3.5 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 FITC Glucose Oxidase Gold 12nm Gold 6nm HRP HRP polymer IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated

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    Overview

    • Product name

      Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed
      See all IgG secondary antibodies
    • Host species

      Goat
    • Target species

      Rabbit
    • Specificity

      By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.

       

    • Tested applications

      Suitable for: IHC-P, ICC/IF, Flow Cytmore details
    • Minimal
      cross-reactivity


      Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat
      To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
      more details
    • Conjugation

      DyLight® 650. Ex: 654nm, Em: 673nm

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • General notes

      DyLight® 650 replaces DyLight® 649.
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Rabbit
      • IgG
      • Fluorophore
      • DyLight® 650

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab96902 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/50 - 1/500.

    DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.

    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.
    Notes
    IHC-P
    1/50 - 1/500.

    DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.

    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.

    Images

    • Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96902)
      Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96902)
      Emission spectra of DyLight® fluorochromes available in our catalog.
      Line colors represent the approximate visible colors of the wavelength maxima.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (9)

    Publishing research using ab96902? Please let us know so that we can cite the reference in this datasheet.

    ab96902 has been referenced in 9 publications.

    • Thadi A  et al. Molecular Targeting of H/MDM-2 Oncoprotein in Human Colon Cancer Cells and Stem-like Colonic Epithelial-derived Progenitor Cells. Anticancer Res 41:27-42 (2021). PubMed: 33419797
    • Kelesidis T  et al. The ApoA-I mimetic peptide 4F attenuates in vitro replication of SARS-CoV-2, associated apoptosis, oxidative stress and inflammation in epithelial cells. Virulence 12:2214-2227 (2021). PubMed: 34494942
    • Daneshvar N  et al. Premature satellite cell activation before injury accelerates myogenesis and disrupts neuromuscular junction maturation in regenerating muscle. Am J Physiol Cell Physiol 319:C116-C128 (2020). PubMed: 32374678
    • Godinho BMDC  et al. Transvascular Delivery of Hydrophobically Modified siRNAs: Gene Silencing in the Rat Brain upon Disruption of the Blood-Brain Barrier. Mol Ther 26:2580-2591 (2018). PubMed: 30143435
    • Hirukawa M  et al. Development of a Tissue-Engineered Artificial Ligament: Reconstruction of Injured Rabbit Medial Collateral Ligament With Elastin-Collagen and Ligament Cell Composite Artificial Ligament. Artif Organs 42:736-745 (2018). PubMed: 29660790
    • Cox D & Ecroyd H The small heat shock proteins aB-crystallin (HSPB5) and Hsp27 (HSPB1) inhibit the intracellular aggregation of a-synuclein. Cell Stress Chaperones 22:589-600 (2017). PubMed: 28337642
    • Romanelli D  et al. Roles and regulation of autophagy and apoptosis in the remodelling of the lepidopteran midgut epithelium during metamorphosis. Sci Rep 6:32939 (2016). IF . PubMed: 27609527
    • Shenkman M  et al. A shared endoplasmic reticulum-associated degradation pathway involving the EDEM1 protein for glycosylated and nonglycosylated proteins. J Biol Chem 288:2167-78 (2013). ICC/IF . PubMed: 23233672
    • Mehmood R  et al. Cross-talk between distinct nuclear import pathways enables efficient nuclear import of E47 in conjunction with its partner transcription factors. Mol Biol Cell 22:3715-24 (2011). ICC/IF . PubMed: 21832153

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Question

    Phone call regarding IHC-P staining of human samples with ab75850. Observing faint staining with background. Had been using 1/100 overnight at 4oC. Wants to optimise the protocol for double staining with ab377994.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 24 2011

    Answer

    As discussed over the phone I have looked at the protocols we have for double staining to see if I can suggest anything further to our conversation. As discussed the protocol that we have on our website is relatively simple, with sequential incubation of the primary antibodies with individual blocking and washing steps between each incubation. https://www.abcam.com/index.html?pageconfig=resource&rid=11458 A protocol from IHC world recommends much the same: http://www.ihcworld.com/_protocols/general_IHC/immunofl_double_squential.htm As discussed I would initially optimise each of your staining individually and once you are happy with them combine the two protocols. It may be that you experience some non-specific staining due to the two primary antibodies being from the same species. This may be optimised with the blocking steps used. Alternatively the use of unconjugated Fab antibody fragments may be employed, either to "change the species" of one of the primary antibodies or to block the free sites of the secondary antibody first applied. If you would like more detail on how to apply this I can send you the protocols where this is described more fully. As for the staining you are currently performing with ab75850, if you do not see an improvement with the suggestions discussed with the positive control tissue please do let me know.

    Read More

    Abcam Scientific Support

    Answered on Nov 24 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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