Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96902)
Key features and details
- Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Related conjugates and formulations
Overview
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Product name
Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
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Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details -
Conjugation
DyLight® 650. Ex: 654nm, Em: 673nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
DyLight® 650 replaces DyLight® 649. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/50 - 1/500.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
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| ICC/IF |
1/50 - 1/500.
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| Flow Cyt |
1/50 - 1/200.
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| Notes |
|---|
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IHC-P
1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
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ICC/IF
1/50 - 1/500. |
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Flow Cyt
1/50 - 1/200. |
Images
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Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96902)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab96902 has been referenced in 9 publications.
- Thadi A et al. Molecular Targeting of H/MDM-2 Oncoprotein in Human Colon Cancer Cells and Stem-like Colonic Epithelial-derived Progenitor Cells. Anticancer Res 41:27-42 (2021). PubMed: 33419797
- Kelesidis T et al. The ApoA-I mimetic peptide 4F attenuates in vitro replication of SARS-CoV-2, associated apoptosis, oxidative stress and inflammation in epithelial cells. Virulence 12:2214-2227 (2021). PubMed: 34494942
- Daneshvar N et al. Premature satellite cell activation before injury accelerates myogenesis and disrupts neuromuscular junction maturation in regenerating muscle. Am J Physiol Cell Physiol 319:C116-C128 (2020). PubMed: 32374678
- Godinho BMDC et al. Transvascular Delivery of Hydrophobically Modified siRNAs: Gene Silencing in the Rat Brain upon Disruption of the Blood-Brain Barrier. Mol Ther 26:2580-2591 (2018). PubMed: 30143435
- Hirukawa M et al. Development of a Tissue-Engineered Artificial Ligament: Reconstruction of Injured Rabbit Medial Collateral Ligament With Elastin-Collagen and Ligament Cell Composite Artificial Ligament. Artif Organs 42:736-745 (2018). PubMed: 29660790
- Cox D & Ecroyd H The small heat shock proteins aB-crystallin (HSPB5) and Hsp27 (HSPB1) inhibit the intracellular aggregation of a-synuclein. Cell Stress Chaperones 22:589-600 (2017). PubMed: 28337642
- Romanelli D et al. Roles and regulation of autophagy and apoptosis in the remodelling of the lepidopteran midgut epithelium during metamorphosis. Sci Rep 6:32939 (2016). IF . PubMed: 27609527
- Shenkman M et al. A shared endoplasmic reticulum-associated degradation pathway involving the EDEM1 protein for glycosylated and nonglycosylated proteins. J Biol Chem 288:2167-78 (2013). ICC/IF . PubMed: 23233672
- Mehmood R et al. Cross-talk between distinct nuclear import pathways enables efficient nuclear import of E47 in conjunction with its partner transcription factors. Mol Biol Cell 22:3715-24 (2011). ICC/IF . PubMed: 21832153