Goat Anti-Rabbit IgG H&L (Glucose Oxidase) (ab136782)
Key features and details
- Goat Anti-Rabbit IgG H&L (Glucose Oxidase)
- Conjugation: Glucose Oxidase
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, WB, ICC, IHC-P, IHC-Fr
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Overview
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Product name
Goat Anti-Rabbit IgG H&L (Glucose Oxidase)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Tested applications
Suitable for: ELISA, WB, ICC, IHC-P, IHC-Frmore details -
Minimal
cross-reactivity
Human more details -
Immunogen
Highly purified whole rabbit immunoglobulin.
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Conjugation
Glucose Oxidase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituents: 50% Glycerol, 50% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab136782 has been purified using a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
1/100 - 1/1000. Substrate:
10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm.
|
|
| WB |
1/400.
|
|
| ICC |
1/10 - 1/100. Substrate:
15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.
|
|
| IHC-P |
1/10 - 1/100.
|
|
| IHC-Fr |
1/10 - 1/100.
|
| Notes |
|---|
|
ELISA
1/100 - 1/1000. Substrate: 10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm. |
|
WB
1/400. |
|
ICC
1/10 - 1/100. Substrate: 15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness. |
|
IHC-P
1/10 - 1/100. |
|
IHC-Fr
1/10 - 1/100. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab136782 has not yet been referenced specifically in any publications.