Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)
Key features and details
- Goat Anti-Rabbit IgG H&L (HRP polymer)
- Conjugation: HRP polymer
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, Immunoelectrophoresis
Related conjugates and formulations
Overview
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Product name
Goat Anti-Rabbit IgG H&L (HRP polymer)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
Based on IEP, ab214880 reacts with heavy (gamma) chains on rabbit IgG, light chains on all rabbit immunoglobulins. No cross-reactivity is observed to non-immunoglobulin rabbit serum proteins, serum proteins from human, mouse or rat. -
Tested applications
Suitable for: IHC-P, Immunoelectrophoresismore details -
Immunogen
Full length protein corresponding to Rabbit IgG. (Purified Rabbit IgG, whole molecule).
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Conjugation
HRP polymer
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Proclin 300
Constituents: 1% BSA, 98% PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
ab214880 was affinity purified using solid phase Rabbit IgG. Purity >95% by SDS-PAGE. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (3) |
Use at an assay dependent concentration.
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| Immunoelectrophoresis |
Use at an assay dependent concentration.
This product is provided in a pre-diluted, ready-to-use format. Incubate 150 µl per slide for 1 hour. |
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
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Immunoelectrophoresis
Use at an assay dependent concentration. This product is provided in a pre-diluted, ready-to-use format. Incubate 150 µl per slide for 1 hour. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)Sonzogni et al PLoS Biol. 2018 Jun 20;16(6):e2004049. doi: 10.1371/journal.pbio.2004049. eCollection 2018 Jun. Fig S2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Representative images IHC for Ki67 (upper panel) and CC3 (lower panel); scale bar 50 μm.
The paraffin sections were deparaffinized and rehydrated, followed by antigen retrieval using sodium citrate buffer (pH 6). The sections were then incubated with 3% hydrogen peroxide to block endogenous peroxidase activity for 10 minutes at room temperature. After 3 washes with TBS, the sections were incubated with 2% bovine serum albumin for 1 hour at room temperature.
Slides were then incubated separately with rabbit anti-Ki67 and rabbit anti-cleaved caspase 3 overnight at 4°C. The slides were washed 3 times and incubated with goat anti-rabbit HRP polymer (1:1, Abcam ab214880) for 2 hours at room temperature. Samples were washed thrice and then developed, counterstained with hematoxylin and then dehydrated before mounting the slides with permount.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880)Immunohistochemical analysis of ER/PR positive breast cancer tissue stained for ER and detected with ab214880.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (37)
ab214880 has been referenced in 37 publications.
- Linke F et al. Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging. Acta Neuropathol Commun 11:6 (2023). PubMed: 36631900
- Nikolos F et al. Cell death-induced immunogenicity enhances chemoimmunotherapeutic response by converting immune-excluded into T-cell inflamed bladder tumors. Nat Commun 13:1487 (2022). PubMed: 35347124
- van Loon K et al. Cancer Vaccination against Extracellular Vimentin Efficiently Adjuvanted with Montanide ISA 720/CpG. Cancers (Basel) 14:N/A (2022). PubMed: 35681575
- Mazumder S et al. Formulation of an ovarian cancer vaccine with the squalene-based AddaVax adjuvant inhibits the growth of murine epithelial ovarian carcinomas. Clin Exp Vaccine Res 11:163-172 (2022). PubMed: 35799868
- Tonnus W et al. Gasdermin D-deficient mice are hypersensitive to acute kidney injury. Cell Death Dis 13:792 (2022). PubMed: 36109515