Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
Key features and details
- Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed
- Conjugation: IRDye® 800CW
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, In-Cell ELISA
Related conjugates and formulations
Overview
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Product name
Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Tested applications
Suitable for: WB, In-Cell ELISAmore details -
Minimal
cross-reactivity
Chicken, Human, Mouse, Rat, Sheep more details -
Immunogen
Full length native protein (purified) corresponding to Rabbit IgG.
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Conjugation
IRDye® 800CW
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS
Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer. -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Affinity chromatography purified using pooled rabbit IgG covalently linked to agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000 - 1/25000.
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| In-Cell ELISA | (1) |
1/200 - 1/1200.
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| Notes |
|---|
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WB
1/5000 - 1/25000. |
|
In-Cell ELISA
1/200 - 1/1200. |
Images
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Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)All lanes : Anti-NF-kB p65 antibody (ab16502) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : NF-kB p65 knockout HAP1 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilutionThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab16502 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Merged signal (red and green). Green - ab16502 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776.
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Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)All lanes : Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1 µg/ml
Lane 1 : Wild-type HAP1 nuclear lysate
Lane 2 : Lamin B1 knockout HAP1 nuclear lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Performed under reducing conditions.This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab16048 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Merged signal (red and green). Green signal from target - ab16048 observed at 68 kDa. Red signal from loading control - ab10799 ( (loading control to histone H3 at 0.1µg/ml) observed at 18 kDa using as secondary Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776.
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Spectrum - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)Excitation and emission spectrum of IRDye® 800CW Ex: 776nm, Em: 792nm.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (66)
ab216773 has been referenced in 66 publications.
- Snetkov X et al. A Conserved Tryptophan in the Envelope Cytoplasmic Tail Regulates HIV-1 Assembly and Spread. Viruses 14:N/A (2022). PubMed: 35062333
- David A et al. A common TMPRSS2 variant has a protective effect against severe COVID-19. Curr Res Transl Med 70:103333 (2022). PubMed: 35104687
- Geron I et al. An instructive role for Interleukin-7 receptor α in the development of human B-cell precursor leukemia. Nat Commun 13:659 (2022). PubMed: 35115489
- Hao X et al. MPZ gene variant site in Chinese patients with Charcot-Marie-Tooth disease. Mol Genet Genomic Med 10:e1890 (2022). PubMed: 35174662
- Ge Y et al. Alpha-Asaronol Alleviates Dysmyelination by Enhancing Glutamate Transport Through the Activation of PPARγ-GLT-1 Signaling in Hypoxia-Ischemia Neonatal Rats. Front Pharmacol 13:766744 (2022). PubMed: 35401225