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Goat Anti-Rabbit IgG H&L (PE) preadsorbed (ab72465)

Reviews (1)Q&A (5)References (10)
Flow Cytometry - Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)
  • Sandwich ELISA - Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)

Key features and details

  • Goat Anti-Rabbit IgG H&L (PE) preadsorbed
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: Flow Cyt, ELISA

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy3.5 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 12nm Gold 6nm HRP HRP polymer IRDye® 800CW Texas Red ® TRITC Unconjugated Unconjugated

Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (PE) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ELISAmore details

  • Minimal
    cross-reactivity


    Cow, Mouse more details

  • Immunogen

    Rabbit IgG

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.71% Sucrose, 0.27% sodium dihydrogen phosphate, 1.09% disodium hydrogen phosphate
  • Concentration information loading...

  • Purity

    Size exclusion

  • Purification notes

    Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.

  • Conjugation notes

    Excitation max. l 565>498 nm. Emission max. l 578 nm. F/P 0.8:1.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Associated products

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab72465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (1)
1/500 - 1/1000.
ELISA
Use at an assay dependent concentration.
Notes
Flow Cyt
1/500 - 1/1000.
ELISA
Use at an assay dependent concentration.

Images

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)
    Flow Cytometry - Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)

    Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Goat anti-rabbit IgG H&L (Phycoerythrin, pre-adsorbed) (ab72465) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 575/30 bandpass filter.

  • Sandwich ELISA - Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)
    Sandwich ELISA - Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)
    Sandwich ELISA to detect Rabbit IgG. Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated Rabbit IgG and read after a 2 hour incubation at room temperature with ab72465

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (10)

Publishing research using ab72465? Please let us know so that we can cite the reference in this datasheet.

ab72465 has been referenced in 10 publications.

  • Gamal-Eldeen AM  et al. Anti-hypoxic Effect of Polysaccharide Extract of Brown Seaweed Sargassum dentifolium in Tongue Squamous Cell Carcinoma. Front Nutr 9:854780 (2022). PubMed: 35399691
  • Watson AW  et al. Breast tumor stiffness instructs bone metastasis via maintenance of mechanical conditioning. Cell Rep 35:109293 (2021). PubMed: 34192535
  • Hwang HJ  et al. The BTLA and PD-1 signaling pathways independently regulate the proliferation and cytotoxicity of human peripheral blood ?d T cells. Immun Inflamm Dis 9:274-287 (2021). PubMed: 33332777
  • Mutoh T  et al. Identification of two major autoantigens negatively regulating endothelial activation in Takayasu arteritis. Nat Commun 11:1253 (2020). PubMed: 32152303
  • Guo C  et al. Loss of FGFR3 Delays Acute Myeloid Leukemogenesis by Programming Weakly Pathogenic CD117-Positive Leukemia Stem-Like Cells. Front Pharmacol 11:632809 (2020). PubMed: 33584313
  • Lin W  et al. Sirtuin4 suppresses the anti-neuroinflammatory activity of infiltrating regulatory T cells in the traumatically injured spinal cord. Immunology 158:362-374 (2019). PubMed: 31559637
  • Holm LJ  et al. Fenofibrate increases very-long-chain sphingolipids and improves blood glucose homeostasis in NOD mice. Diabetologia N/A:N/A (2019). PubMed: 31410530
  • Castellini C  et al. In vitro effect of nerve growth factor on the main traits of rabbit sperm. Reprod Biol Endocrinol 17:93 (2019). PubMed: 31718673
  • Volpetti F  et al. A microfluidic platform for high-throughput multiplexed protein quantitation. PLoS One 10:e0117744 (2015). PubMed: 25680117
  • Morris D  et al. Glutathione synthesis is compromised in erythrocytes from individuals with HIV. Front Pharmacol 5:73 (2014). PubMed: 24782776

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

detection of mouse erythrocyte surface proteins by flow cytometry

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
This secondary antibody was used to detect Slc4a1 on mouse erythrocytes as control as well as differentiated mouse embryonic stem cells. It can be seen in the picture attached that the labelling detected with this secondary antibody alone is practically identical to that obtained with un-labelled cells, meaning that the background is very low, which increases the confidence in the specificity of the primary antibody used.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Alena Pance

Verified customer

Submitted Sep 08 2014

Question

Email: iisakova@tulane.edu Please let me know whether I can use this antibody for the immuno-cytochemistry. I bought it to use in flow cytometry, but now need to stain 4% PFD-fixed cells on plastic. Thanks.

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Answer

Thank you for contacting Abcam.

This product has not been tested in ICC and so while we cannot guarantee that it will work in that application, we see no reason to believe that it wouldn't. We would of course recommend thoroughly blocking your samples to minimize any non-specific binding in a sandard ICC blocking solution containing BSA or a 10% rabbit serum.

I hope this information helps. Please let me know if you have any questions.

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Question

Customer kindly contacted us to inquire about the immunogen sequence for ab56215.

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Answer

Thank you for contacting us today regarding secondary antibodies.

I have made a short list of compatible antibodies for use withab56215 Anti-SLC4A4 antibody.These products are pre-absorbed to ensure minimum cross reactivity. Each product is conjugated a different fluorochome so that you may chose the one which best fits your experiments. Please let me know if you have any questions.

AMCA:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-AMCA-ab123435.html

PE:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-PE-pre-adsorbed-ab72465.html

DyLight 594:https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-594-pre-adsorbed-ab98500.html

Texas Red:https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-Pre-Adsorbed-ab7088.html

APC:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-Allophycocyanin-pre-adsorbed-ab130805.html

If testing live cells we also recommend cell viability controls such as 7-AAD orPropidium Iodide.Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. We do carry PI and offer a 7-AADCell-Mediated Cytotoxicity Assay Kit.

Propidium Iodide: https://www.abcam.com/Propidium-Iodide-ab14083.html

7-AAD Kit:https://www.abcam.com/7-AADCFSE-Cytotoxicity-Assay-Kit-ab133073.html



In reviewing the datasheet for this product I did note that it has not been tested in Flow Cytometry. As such we cannot guarantee ab56212 in that application. However by participating in our AbTrial program, you can now use our products in an untested application or species without financial risk.



Simply follow these easy steps below to take part in our AbTrial Program:

1. Reply to this e-mail to let me know that you would like to proceed and test this product in Flow Cytometry.

2. I will email you aninactivepersonal discount code for the value of the product.

3. Purchase and test the product at regular price.

4. https://www.abcam.com/abreviews with you results noting your AbTrial discount code in the additional notes section.

5. Once the Abreview is submitted, the discount code will becomeactive.

6. Apply your discount code on your next order to receive that value off.

More information including the terms and conditions about this program may be found at: https://www.abcam.com/AbTrial

I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.

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Question

I forgot to mention that I am going to use them for flow cytometry (on LSR II).

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Answer

Thank you for your reply.
Below are the 2 products that I would recommend for you:
Isotype control - https://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html
Secondary - https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-PE-pre-adsorbed-ab97070.html
Both of these are covered under our Abpromise and are guaranteed to work in Flow Cytometry.
If there is anything else I can help you with, please let me know.

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Question

I am looking for an isotype control and a conjugated secondary antibody fora this primary antibody of Cyclin E, https://www.abcam.com/Cyclin-E-antibody-ab7959.html It seems that you have the following relevant products for instance: https://www.abcam.com/Rabbit-polyclonal-IgG-ab27472.html https://www.abcam.com/Rabbit-polyclonal-IgG-ab27478.html https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-PE-pre-adsorbed-ab72465.html https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-PE-pre-adsorbed-ab97070.html Please let me know which products you would recommend most for best results.

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Answer

Thank you for contacting Abcam.
I would be happy to help you select the best products for your experiments. To be able to assist you further, would you please let me know which applications you wish to use these products in. That way I can recommend products that have been tested and are guaranteed to work in particular applications.
I look forward to your reply.
Also, for the month of Spetember we are running a buy 4 for 3 promotion, purhcase any 3 products and get a 4th free. If you wich to take advantage of this promotion, just place the code ** in your next order.

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Question

Dear Sirt’s Reference to the above subject (Glucocorticoid Receptor; ab9568 or ab109022) .. the customer need to work it by Flow Cytometer.. and I think this type of work need a secondary anti-body. But the TDS (technical data sheet) on the abcam website doesn’t suggest any kind of secondary anti-body. So please suggest a secondary anti-body by color of PE (Phycoerythrin) work with ab9568 or ab109022 by Flow Cytometer. thanks Sincerely yours,      

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Answer

Thank you for your enquiry and interest in our products. In order to be able to search the compatible secondary antibodies, first  it is important to consider the isotypes of these two primary antibodies: ab9568 - mouse IgG1 kappa ab109022 - rabbit IgG1 I would advise you and your customer to use our recently developed advanced search facilities for secondary antibodies: https://www.abcam.com/index.html?pageconfig=productmap&cl=918 I have conducted some search for you and you can find some compatible antibodies: For ab9568: ab99605 - Rat monoclonal [SB77e] Secondary Antibody to Mouse IgG1 (PE), pre-adsorbed ab99912 - Goat polyclonal Secondary Antibody to Mouse IgG1 (PE) For ab109022: ab97070 - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed -Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), ab98441- Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), ab98494- Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed, ab7007- Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed, ab72465 - Goat polyclonal Secondary Antibody to Rabbit IgG (PE), pre-adsorbed   I hope this helps and if I can assist further, please do not hesitate to contact me.    

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