Goat Anti-Rabbit IgG H&L (PE) preadsorbed (ab72465)
Key features and details
- Goat Anti-Rabbit IgG H&L (PE) preadsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ELISA
Related conjugates and formulations
Overview
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Product name
Goat Anti-Rabbit IgG H&L (PE) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ELISAmore details -
Minimal
cross-reactivity
Cow, Mouse more details -
Immunogen
Rabbit IgG
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Conjugation
PE. Ex: 488nm, Em: 575nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.71% Sucrose, 0.27% sodium dihydrogen phosphate, 1.09% disodium hydrogen phosphate -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used. -
Conjugation notes
Excitation max. l 565>498 nm. Emission max. l 578 nm. F/P 0.8:1. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (1) |
1/500 - 1/1000.
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| ELISA |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Flow Cyt
1/500 - 1/1000. |
|
ELISA
Use at an assay dependent concentration. |
Images
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Flow Cytometry - Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)
Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Goat anti-rabbit IgG H&L (Phycoerythrin, pre-adsorbed) (ab72465) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 575/30 bandpass filter.
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Sandwich ELISA - Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)Sandwich ELISA to detect Rabbit IgG. Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated Rabbit IgG and read after a 2 hour incubation at room temperature with ab72465
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab72465 has been referenced in 15 publications.
- Gouhar SA et al. Involvement of miRNAs in response to oxidative stress induced by the steroidal glycoalkaloid α-solanine in hepatocellular carcinoma cells. Environ Toxicol 37:212-223 (2022). PubMed: 34655286
- Derkus B et al. Xenogenic Neural Stem Cell-Derived Extracellular Nanovesicles Modulate Human Mesenchymal Stem Cell Fate and Reconstruct Metabolomic Structure. Adv Biol (Weinh) 6:e2101317 (2022). PubMed: 35347890
- Qiang P et al. Esaxerenone inhibits the macrophage-to-myofibroblast transition through mineralocorticoid receptor/TGF-β1 pathway in mice induced with aldosterone. Front Immunol 13:948658 (2022). PubMed: 36148244
- Kuhn MR et al. Myeloid cell-derived catecholamines influence bone turnover and regeneration in mice. Front Endocrinol (Lausanne) 13:997745 (2022). PubMed: 36187089
- Gamal-Eldeen AM et al. Anti-hypoxic Effect of Polysaccharide Extract of Brown Seaweed Sargassum dentifolium in Tongue Squamous Cell Carcinoma. Front Nutr 9:854780 (2022). PubMed: 35399691