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    products/secondary-antibodies/goat-rabbit-igg-hl-pe-preadsorbed-ab72465.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rabbit IgG H&L (PE) preadsorbed (ab72465)

  • Datasheet
  • SDS
Reviews (1)Q&A (5)References (15)

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Flow Cytometry - Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)
  • Sandwich ELISA - Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)

Key features and details

  • Goat Anti-Rabbit IgG H&L (PE) preadsorbed
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: Flow Cyt, ELISA

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy3.5 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 12nm Gold 6nm HRP HRP polymer IRDye® 800CW Texas Red ® TRITC Unconjugated Unconjugated

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Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (PE) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity


    Cow, Mouse
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    Rabbit IgG

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.71% Sucrose, 0.27% sodium dihydrogen phosphate, 1.09% disodium hydrogen phosphate
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.
  • Conjugation notes

    Excitation max. l 565>498 nm. Emission max. l 578 nm. F/P 0.8:1.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Fluorophore
    • Phycoerythrin

Associated products

  • Related Products

    • Anti-CD3 antibody [SP7] (ab16669)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab72465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (1)
1/500 - 1/1000.
ELISA
Use at an assay dependent concentration.
Notes
Flow Cyt
1/500 - 1/1000.
ELISA
Use at an assay dependent concentration.

Images

  • Flow Cytometry - Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)
    Flow Cytometry - Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)

    Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Goat anti-rabbit IgG H&L (Phycoerythrin, pre-adsorbed) (ab72465) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 575/30 bandpass filter.

  • Sandwich ELISA - Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)
    Sandwich ELISA - Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)
    Sandwich ELISA to detect Rabbit IgG. Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated Rabbit IgG and read after a 2 hour incubation at room temperature with ab72465

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (15)

Publishing research using ab72465? Please let us know so that we can cite the reference in this datasheet.

ab72465 has been referenced in 15 publications.

  • Gouhar SA  et al. Involvement of miRNAs in response to oxidative stress induced by the steroidal glycoalkaloid α-solanine in hepatocellular carcinoma cells. Environ Toxicol 37:212-223 (2022). PubMed: 34655286
  • Derkus B  et al. Xenogenic Neural Stem Cell-Derived Extracellular Nanovesicles Modulate Human Mesenchymal Stem Cell Fate and Reconstruct Metabolomic Structure. Adv Biol (Weinh) 6:e2101317 (2022). PubMed: 35347890
  • Qiang P  et al. Esaxerenone inhibits the macrophage-to-myofibroblast transition through mineralocorticoid receptor/TGF-β1 pathway in mice induced with aldosterone. Front Immunol 13:948658 (2022). PubMed: 36148244
  • Kuhn MR  et al. Myeloid cell-derived catecholamines influence bone turnover and regeneration in mice. Front Endocrinol (Lausanne) 13:997745 (2022). PubMed: 36187089
  • Gamal-Eldeen AM  et al. Anti-hypoxic Effect of Polysaccharide Extract of Brown Seaweed Sargassum dentifolium in Tongue Squamous Cell Carcinoma. Front Nutr 9:854780 (2022). PubMed: 35399691
View all Publications for this product

Customer reviews and Q&As

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detection of mouse erythrocyte surface proteins by flow cytometry

Excellent
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
This secondary antibody was used to detect Slc4a1 on mouse erythrocytes as control as well as differentiated mouse embryonic stem cells. It can be seen in the picture attached that the labelling detected with this secondary antibody alone is practically identical to that obtained with un-labelled cells, meaning that the background is very low, which increases the confidence in the specificity of the primary antibody used.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Alena Pance

Verified customer

Submitted Sep 08 2014

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