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Goat Anti-Rabbit IgG H&L preadsorbed (ab7085)

Submit a review Q&A (1)References (2)

Key features and details

  • Goat Anti-Rabbit IgG H&L preadsorbed
  • Host species: Goat
  • Suitable for: ICC/IF, WB, IHC-P, IP, Immunodiffusion, Conjugation, IHC-Fr, ELISA

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Overview

  • Product name

    Goat Anti-Rabbit IgG H&L preadsorbed
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, IP, Immunodiffusion, Conjugation, IHC-Fr, ELISAmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details

  • Immunogen

    Full length native Rabbit IgG (purified).

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.424% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.

  • Clonality

    Polyclonal

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/500 - 1/2000.
WB
1/500 - 1/2000.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/270000.
Notes
ICC/IF
1/500 - 1/2000.
WB
1/500 - 1/2000.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/270000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (2)

Publishing research using ab7085? Please let us know so that we can cite the reference in this datasheet.

ab7085 has been referenced in 2 publications.

  • Wang X  et al. Insufficient radiofrequency ablation promotes hepatocellular carcinoma cell progression via autophagy and the CD133 feedback loop. Oncol Rep 40:241-251 (2018). PubMed: 29749472
  • Xiong Y  et al. STAT3-regulated long non-coding RNAs lnc-7SK and lnc-IGF2-AS promote hepatitis C virus replication. Mol Med Rep 12:6738-44 (2015). PubMed: 26328522

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Inquiry: Hi, we recently got your Goat polyclonal secondary antibody to rabbit IgG- H&L, pre-adsorbed, ab7085. The datasheet states that the storage buffer contains: 0.01% sodium azide as a presevative and further more 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2. As we want to conjugate this antibody ourselves, the very high BSA content is a problem. Thus, we purified the Abs using a IgG binding column. After this we evaluated the fractions by SDS-PAGE. We could not observe any BSA, not even in the unpurified sample (stated to contain 10 times as much BSA compared to goat pAb). The question is then, is there an error in your Datasheet? Or is there an error somewhere else? Did we obtain the correct antibody? It would be very nice if you could just confirm if there's a problem in the datasheet, or what else could be the cause for us not detecting any BSA in our sample.

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Answer

Thank you for contacting us.

I have been in contact with the lab and they have double checked the storage buffer. I can confirm that ab7085 does not contain any BSA. I am very sorry for this error on our part and I appreciate the time and effort you have gone to to remove BSA from our product.

I would like to thank you for pointing this out to us as it will allow us to update our datasheets.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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