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  1. Link

    products/secondary-antibodies/goat-rabbit-igg-hl-preadsorbed-ab7085.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rabbit IgG H&L preadsorbed (ab7085)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (2)

Product price, shipping and contact information

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Goat Anti-Rabbit IgG H&L preadsorbed
  • Host species: Goat
  • Suitable for: ICC/IF, WB, IHC-P, IP, Immunodiffusion, Conjugation, IHC-Fr, ELISA

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy3.5 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 12nm Gold 6nm HRP HRP polymer IRDye® 800CW PE Texas Red ® TRITC Unconjugated

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Overview

  • Product name

    Goat Anti-Rabbit IgG H&L preadsorbed
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, IP, Immunodiffusion, Conjugation, IHC-Fr, ELISAmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    Full length native Rabbit IgG (purified).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.424% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • Clonality

    Polyclonal
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Unconjugated

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/500 - 1/2000.
WB
1/500 - 1/2000.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/270000.
Notes
ICC/IF
1/500 - 1/2000.
WB
1/500 - 1/2000.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Immunodiffusion
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/270000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab7085? Please let us know so that we can cite the reference in this datasheet.

ab7085 has been referenced in 2 publications.

  • Wang X  et al. Insufficient radiofrequency ablation promotes hepatocellular carcinoma cell progression via autophagy and the CD133 feedback loop. Oncol Rep 40:241-251 (2018). PubMed: 29749472
  • Xiong Y  et al. STAT3-regulated long non-coding RNAs lnc-7SK and lnc-IGF2-AS promote hepatitis C virus replication. Mol Med Rep 12:6738-44 (2015). PubMed: 26328522

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Inquiry: Hi, we recently got your Goat polyclonal secondary antibody to rabbit IgG- H&L, pre-adsorbed, ab7085. The datasheet states that the storage buffer contains: 0.01% sodium azide as a presevative and further more 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2. As we want to conjugate this antibody ourselves, the very high BSA content is a problem. Thus, we purified the Abs using a IgG binding column. After this we evaluated the fractions by SDS-PAGE. We could not observe any BSA, not even in the unpurified sample (stated to contain 10 times as much BSA compared to goat pAb). The question is then, is there an error in your Datasheet? Or is there an error somewhere else? Did we obtain the correct antibody? It would be very nice if you could just confirm if there's a problem in the datasheet, or what else could be the cause for us not detecting any BSA in our sample.

Read More

Abcam community

Verified customer

Asked on Nov 29 2012

Answer

Thank you for contacting us.

I have been in contact with the lab and they have double checked the storage buffer. I can confirm that ab7085 does not contain any BSA. I am very sorry for this error on our part and I appreciate the time and effort you have gone to to remove BSA from our product.

I would like to thank you for pointing this out to us as it will allow us to update our datasheets.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

Answered on Nov 29 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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