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Goat Anti-Rabbit IgG H&L (Texas Red ®) (ab6719)

Submit a review Q&A (1)References (47)

Key features and details

  • Goat Anti-Rabbit IgG H&L (Texas Red ®)
  • Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, IHC-Fr, Immunomicroscopy, ELISA

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Goat Anti-Rabbit IgG H&L (Texas Red ®)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-P, IHC-Fr, Immunomicroscopy, ELISAmore details

  • Immunogen

    Rabbit IgG, whole molecule

  • Conjugation

    Texas Red ®. Ex: 596nm, Em: 620nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.

  • Conjugation notes

    Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.7 moles Texas Red per mole of Goat IgG

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/1000 - 1/5000.
Flow Cyt
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.
Notes
ICC/IF
1/1000 - 1/5000.
Flow Cyt
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (47)

Publishing research using ab6719? Please let us know so that we can cite the reference in this datasheet.

ab6719 has been referenced in 47 publications.

  • Matthaiou EI  et al. Macrophage Lysosomal Alkalinization Drives Invasive Aspergillosis in a Mouse Cystic Fibrosis Model of Airway Transplantation. J Fungi (Basel) 8:N/A (2022). PubMed: 35887506
  • Ridlo MR  et al. The Improvement of Porcine In Vitro Embryo Development through Regulating Autophagy by miRNA-143 Inhibition. Animals (Basel) 12:N/A (2022). PubMed: 36230392
  • Yardim A  et al. Protective Effects of Curcumin Against Paclitaxel-Induced Spinal Cord and Sciatic Nerve Injuries in Rats. Neurochem Res 46:379-395 (2021). PubMed: 33201400
  • Gharenaz NM  et al. Comparison of two methods for prolong storage of decellularized mouse whole testis for tissue engineering application: An experimental study. Int J Reprod Biomed 19:321-332 (2021). PubMed: 33997591
  • Kim EH  et al. Crosstalk between Peroxisomal Activities and Nrf2 Signaling in Porcine Embryos. Antioxidants (Basel) 10:N/A (2021). PubMed: 34068072
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a question

Question

Hi, Do you know exact position of TR conjugate on antibody structure? I mean does it always bind a specific part of antibody or bind any where on the antibody? As antibody orientation on the gold surface is random I have to know that antibody conjugation to TR is random or not, as well. Thanks Regards

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Answer

Thank you for your enquiry. I can confirm that ab6719 Rabbit IgG secondary antibody has more than 1 molecule of Texas Red per molecule of IgG. During conjugation these molecules are bound randomly to different sites on the IgG molecules. I hope this information is helpful to you. Please do not hesitate to contact us if you have any further questions.

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