Goat Anti-Rat IgG H&L (Alexa Fluor® 555) (ab150158)
Key features and details
- Goat Anti-Rat IgG H&L (Alexa Fluor® 555)
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt
Related conjugates and formulations
Overview
-
Product name
Goat Anti-Rat IgG H&L (Alexa Fluor® 555)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Tested applications
Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details -
Immunogen
Synthetic peptide corresponding to IgG.
-
Conjugation
Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
-
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| ICC/IF | (1) |
1/200 - 1/1000.
|
| ELISA |
Use at an assay dependent concentration. Use at an assay dependent dilution
|
|
| IHC-P |
Use at an assay dependent concentration. Use at an assay dependent dilution
|
|
| Flow Cyt |
1/2000.
|
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC/IF
1/200 - 1/1000. |
|
ELISA
Use at an assay dependent concentration. Use at an assay dependent dilution |
|
IHC-P
Use at an assay dependent concentration. Use at an assay dependent dilution |
|
Flow Cyt
1/2000. |
Images
-
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) (ab150158)ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150158 Alexa Fluor® 555 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) (ab150158)HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions.The secondary antibody (yellow) was ab150158 Alexa Fluor® 555 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 555) (ab150158)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (14)
ab150158 has been referenced in 14 publications.
- Despot-Slade E et al. The Centromere Histone Is Conserved and Associated with Tandem Repeats Sharing a Conserved 19-bp Box in the Holocentromere of Meloidogyne Nematodes. Mol Biol Evol 38:1943-1965 (2021). PubMed: 33399875
- Bai R et al. Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis. EMBO J 39:e103325 (2020). PubMed: 32510170
- O'Hara JM et al. Generation of protective pneumococcal-specific nasal resident memory CD4+ T cells via parenteral immunization. Mucosal Immunol 13:172-182 (2020). PubMed: 31659300
- Bony BA et al. Ultrasmall Mixed Eu-Gd Oxide Nanoparticles for Multimodal Fluorescence and Magnetic Resonance Imaging of Passive Accumulation and Retention in TBI. ACS Omega 5:16220-16227 (2020). PubMed: 32656444
- Cabañero D et al. Protective role of neuronal and lymphoid cannabinoid CB2 receptors in neuropathic pain. Elife 9:N/A (2020). PubMed: 32687056