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    products/secondary-antibodies/goat-rat-igg-hl-alexa-fluor-647-preadsorbed-ab150167.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150167)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150167)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150167)
  • Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150167)

Key features and details

  • Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, IHC-P, ELISA, Flow Cyt

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 6nm HRP HRP polymer PE PE Texas Red ® TRITC Unconjugated Unconjugated

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Overview

  • Product name

    Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rat
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chain common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, IHC-P, ELISA, Flow Cytmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Human, Mouse, Rabbit, Sheep
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rat
    • IgG
    • Fluorophore
    • Alexa Fluor® 647

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Flow Cyt
1/2000.
Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Flow Cyt
1/2000.

Images

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150167)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150167)
    ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 1µg/ml) overnight at +4°C. The secondary antibody (red) was ab150167 Alexa Fluor® 647 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150167)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150167)
    HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (red) was ab150167 Alexa Fluor® 647 goat anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150167)
    Alexa Fluor® - Goat Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150167)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (29)

Publishing research using ab150167? Please let us know so that we can cite the reference in this datasheet.

ab150167 has been referenced in 29 publications.

  • Gupta D  et al. Fucoidan Inhibition of Osteosarcoma Cells Is Species and Molecular Weight Dependent. Mar Drugs 18:N/A (2020). PubMed: 32046368
  • Huang T  et al. The histone modification reader ZCWPW1 links histone methylation to PRDM9-induced double-strand break repair. Elife 9:N/A (2020). PubMed: 32374261
  • Beaver JT  et al. Cutaneous vaccination ameliorates Zika virus-induced neuro-ocular pathology via reduction of anti-ganglioside antibodies. Hum Vaccin Immunother 16:2072-2091 (2020). PubMed: 32758106
  • Beaver JT  et al. Zika virus-induced neuro-ocular pathology in immunocompetent mice correlates with anti-ganglioside autoantibodies. Hum Vaccin Immunother 16:2092-2108 (2020). PubMed: 32758108
  • Xu J  et al. Lysosomal protein surface expression discriminates fat- from bone-forming human mesenchymal precursor cells. Elife 9:N/A (2020). PubMed: 33044169
View all Publications for this product

Customer reviews and Q&As

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