Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed (ab98420)
Key features and details
- Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, IHC-P
Related conjugates and formulations
Overview
-
Product name
Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details -
Conjugation
DyLight® 488. Ex: 493nm, Em: 518nm
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab98420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/50 - 1/200.
|
|
| ICC/IF |
1/50 - 1/500.
|
|
| IHC-P |
1/50 - 1/500.
|
| Notes |
|---|
|
Flow Cyt
1/50 - 1/200. |
|
ICC/IF
1/50 - 1/500. |
|
IHC-P
1/50 - 1/500. |
Images
-
Spectrum - Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed (ab98420)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima. -
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed (ab98420)ICC/IF image of ab6161 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rat- H&L, pre-adsorbed (ab98420) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (4)
ab98420 has been referenced in 4 publications.
- Yoo B et al. RNAi-Mediated PD-L1 Inhibition for Pancreatic Cancer Immunotherapy. Sci Rep 9:4712 (2019). PubMed: 30886310
- Yoo B et al. MicroRNA-710 regulates multiple pathways of carcinogenesis in murine metastatic breast cancer. PLoS One 14:e0226356 (2019). PubMed: 31834924
- Shrestha RL et al. TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes. Open Biol 4:130108 (2014). ICC/IF . PubMed: 24898139
- Liegel RP et al. Loss-of-function mutations in TBC1D20 cause cataracts and male infertility in blind sterile mice and Warburg micro syndrome in humans. Am J Hum Genet 93:1001-14 (2013). PubMed: 24239381
