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    products/secondary-antibodies/goat-rat-igg-hl-dylight-488-preadsorbed-ab98420.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed (ab98420)

  • Datasheet
Submit a review Submit a question References (4)

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Spectrum - Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed (ab98420)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed (ab98420)

Key features and details

  • Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed
  • Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: Flow Cyt, ICC/IF, IHC-P

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 6nm HRP HRP polymer PE PE Texas Red ® TRITC Unconjugated Unconjugated

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Overview

  • Product name

    Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rat
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rat
    • IgG
    • Fluorophore
    • DyLight® 488

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab98420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/50 - 1/200.
ICC/IF
1/50 - 1/500.
IHC-P
1/50 - 1/500.
Notes
Flow Cyt
1/50 - 1/200.
ICC/IF
1/50 - 1/500.
IHC-P
1/50 - 1/500.

Images

  • Spectrum - Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed (ab98420)
    Spectrum - Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed (ab98420)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed (ab98420)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed (ab98420)

    ICC/IF image of ab6161 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rat- H&L, pre-adsorbed (ab98420) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (4)

Publishing research using ab98420? Please let us know so that we can cite the reference in this datasheet.

ab98420 has been referenced in 4 publications.

  • Yoo B  et al. RNAi-Mediated PD-L1 Inhibition for Pancreatic Cancer Immunotherapy. Sci Rep 9:4712 (2019). PubMed: 30886310
  • Yoo B  et al. MicroRNA-710 regulates multiple pathways of carcinogenesis in murine metastatic breast cancer. PLoS One 14:e0226356 (2019). PubMed: 31834924
  • Shrestha RL  et al. TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes. Open Biol 4:130108 (2014). ICC/IF . PubMed: 24898139
  • Liegel RP  et al. Loss-of-function mutations in TBC1D20 cause cataracts and male infertility in blind sterile mice and Warburg micro syndrome in humans. Am J Hum Genet 93:1001-14 (2013). PubMed: 24239381

Customer reviews and Q&As

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