Goat anti-Rat IgG H&L (PE) Pre-Adsorbed (ab7010)
Key features and details
- Goat anti-Rat IgG H&L (PE) Pre-Adsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt
Related conjugates and formulations
Overview
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Product name
Goat anti-Rat IgG H&L (PE) Pre-Adsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details -
Minimal
cross-reactivity
Cow, Horse, Human, Mouse, Rabbit, Sheep more details -
Immunogen
Full length native protein (purified) corresponding to IgG.
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Conjugation
PE. Ex: 488nm, Em: 575nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Prepared from monospecific antiserum by immunoaffinity chromatography using rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation -
Conjugation notes
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/250.
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| IHC-P |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
|
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| Immunomicroscopy |
1/50 - 1/200.
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| Flow Cyt |
1/100 - 1/250.
Use 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. |
| Notes |
|---|
|
ICC/IF
1/100 - 1/250. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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Immunomicroscopy
1/50 - 1/200. |
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Flow Cyt
1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab7010 has been referenced in 22 publications.
- Jalali Tehrani H et al. Application of adipose-derived, muscle-derived, and co-cultured stem cells for the treatment of stress urinary incontinence in rat models. Low Urin Tract Symptoms 13:308-318 (2021). PubMed: 33098273
- Xu J et al. Discovering the mechanism and involvement of the methylation of cyclin-dependent kinase inhibitor 2A (CDKN2A) gene and its special locus region in gastric cancer. Bioengineered 12:1286-1298 (2021). PubMed: 33896386
- Yang Y et al. 1-Trichloromethyl-1,2,3,4-tetrahydro-beta-carboline (TaClo) Induces the Apoptosis of Dopaminergic Neurons via Oxidative Stress and Neuroinflammation. Oxid Med Cell Longev 2019:1292891 (2019). PubMed: 30984332
- Zeng L et al. Long non-coding RNA MALAT-1 contributes to maintenance of stem cell-like phenotypes in breast cancer cells. Oncol Lett 15:2117-2122 (2018). PubMed: 29434914
- Lin L et al. Cocaine- and amphetamine-regulated transcript (CART) is associated with dopamine and is protective against ischemic stroke. Mol Med Rep 18:3298-3304 (2018). PubMed: 30066844