Goat Anti-Rat IgG H&L (TRITC) preadsorbed (ab7094)
Key features and details
- Goat Anti-Rat IgG H&L (TRITC) preadsorbed
- Conjugation: TRITC. Ex: 547nm, Em: 572nm
- Host species: Goat
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA
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Unconjugated
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Overview
-
Product name
Goat Anti-Rat IgG H&L (TRITC) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Tested applications
Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details -
Immunogen
Full length native Rat IgG (purified).
-
Conjugation
TRITC. Ex: 547nm, Em: 572nm
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 0.02% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7094 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
|
|
| Immunomicroscopy |
Use at an assay dependent dilution.
|
|
| Flow Cyt |
Use at an assay dependent dilution.
|
|
| IHC-P |
Use at an assay dependent dilution.
|
|
| IHC-Fr |
Use at an assay dependent dilution.
|
|
| ELISA |
1/10000 - 1/50000.
|
| Notes |
|---|
|
ICC/IF
1/1000 - 1/5000. |
|
Immunomicroscopy
Use at an assay dependent dilution. |
|
Flow Cyt
Use at an assay dependent dilution. |
|
IHC-P
Use at an assay dependent dilution. |
|
IHC-Fr
Use at an assay dependent dilution. |
|
ELISA
1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (5)
ab7094 has been referenced in 5 publications.
- Cui C et al. Coapplication of Magnesium Supplementation and Vibration Modulate Macrophage Polarization to Attenuate Sarcopenic Muscle Atrophy through PI3K/Akt/mTOR Signaling Pathway. Int J Mol Sci 23:N/A (2022). PubMed: 36361730
- Shimizu T et al. Expression of Francisella pathogenicity island protein intracellular growth locus E (IglE) in mammalian cells is involved in intracellular trafficking, possibly through microtubule organizing center. Microbiologyopen N/A:e00684 (2018). PubMed: 29978561
- Warrington JP et al. Cerebral microvascular rarefaction induced by whole brain radiation is reversible by systemic hypoxia in mice. Am J Physiol Heart Circ Physiol 300:H736-44 (2011). PubMed: 21186274
- Kumar M et al. Novel membrane-permeable contrast agent for brain tumor detection by MRI. Magn Reson Med 63:617-24 (2010). IHC-Fr ; Mouse . PubMed: 20146231
- Medarova Z et al. In vivo imaging of siRNA delivery and silencing in tumors. Nat Med 13:372-377 (2007). PubMed: 17322898
