Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)
![ELISA - Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)](https://www.abcam.com/ps/products/99/ab99818/Images/ab99818-293781-anti-human-igg4-pfcrsquo-hp6023-biotin-elisa.jpg "ELISA - Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)")
Key features and details
- Anti-Human IgG4 pFc’ [HP6023] (Biotin)
- Conjugation: Biotin
- Host species: Mouse
- Isotype: IgG3
- Suitable for: ELISA, Dot blot, ICC/IF, IHC-Fr, WB
Related conjugates and formulations
Overview
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Product name
Anti-Human IgG4 pFc’ [HP6023] (Biotin)
See all IgG4 secondary antibodies -
Host species
Mouse -
Target species
Human -
Specificity
ab99818 reacts with the pFc’ portion of the heavy chain of Human IgG4. -
Tested applications
Suitable for: ELISA, Dot blot, ICC/IF, IHC-Fr, WBmore details -
Immunogen
Full length protein corresponding to Human IgG4. Myeloma protein
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HP6023 -
Isotype
IgG3 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
1/1000 - 1/2000.
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| Dot blot |
Use at an assay dependent dilution. for Dot and slot immunoblotting.
|
|
| ICC/IF |
Use at an assay dependent dilution.
|
|
| IHC-Fr |
Use at an assay dependent dilution.
|
|
| WB |
Use at an assay dependent dilution.
|
| Notes |
|---|
|
ELISA
1/1000 - 1/2000. |
|
Dot blot
Use at an assay dependent dilution. for Dot and slot immunoblotting. |
|
ICC/IF
Use at an assay dependent dilution. |
|
IHC-Fr
Use at an assay dependent dilution. |
|
WB
Use at an assay dependent dilution. |
Images
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ELISA - Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)
ELISA - Anti-Human IgG4 pFc [HP6023] (Biotin) (ab99818)
ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4.
Immunoglobulins were detected with serially diluted ab99818 followed by Streptavidin-HRP.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab99818 has been referenced in 2 publications.
- Tschismarov R et al. Antibody effector analysis of prime versus prime-boost immunizations with a recombinant measles-vectored chikungunya virus vaccine. JCI Insight 6:N/A (2021). PubMed: 34582377
- Boylan B & Miller CH Effects of pre-analytical heat treatment in factor VIII (FVIII) inhibitor assays on FVIII antibody levels. Haemophilia 24:487-491 (2018). PubMed: 29461004