Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)
![ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)](https://www.abcam.com/ps/products/1/ab1927/Images/ab1927-331323-mouse-monoclonal-2c11-anti-human-igg1-fc-elisa.jpg "ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)")
Key features and details
- Mouse monoclonal [2C11] Anti-Human IgG1 Fc
- Host species: Mouse
- Isotype: IgG1
- Suitable for: IHC-Fr, ELISA
Overview
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Product name
Mouse monoclonal [2C11] Anti-Human IgG1 Fc
See all IgG1 secondary antibodies -
Host species
Mouse -
Target species
Human -
Tested applications
Suitable for: IHC-Fr, ELISAmore details -
Immunogen
Full length native protein (purified) corresponding to Human IgG1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Chromatography on protein A Sepharose -
Clonality
Monoclonal -
Clone number
2C11 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
General notes
Concentration varies from lot to lot and can be provided on request. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration. PubMed: 24373342
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. PubMed: 24373342 |
Images
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ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)Marino et al PLoS One. 2017 May 18;12(5):e0177810. doi: 10.1371/journal.pone.0177810. eCollection 2017. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Characterisation of virus activity and functional expression of monoclonal antibodies.
(Panel B) IgG1 antibody expression detected in cellular supernatants of HT-29 cells infected with 10ppc NG-135 by anti-IgG1 ELISA.
Media was removed from infected or control cells, clarified by centrifugation and diluted 1 in 2 in 3% BSA/PBS for storage at -20°C. For quantification of human IgG1, 96 well plates (NUNC) were coated overnight, 4°C, with mouse monoclonal anti-human IgG1 Fc antibody (ab1927, Abcam) at a 1:1000 dilution in carbonate/bicarbonate buffer. Plates were blocked in 3% BSA/PBS, washed and samples and standards added to the plate for 1 hr and RT. Secondary detection was carried out using a HRP conjugated goat anti-human IgG Fab (ab87422, Abcam) incubated for 1 hr, RT.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab1927 has been referenced in 12 publications.
- Van Thillo Q et al. Oncogenic cooperation between TCF7-SPI1 and NRAS(G12D) requires ß-catenin activity to drive T-cell acute lymphoblastic leukemia. Nat Commun 12:4164 (2021). PubMed: 34230493
- Wang J et al. Krüppel like factor 6 splice variant 1 (KLF6-SV1) overexpression recruits macrophages to participate in lung cancer metastasis by up-regulating TWIST1. Cancer Biol Ther 20:680-691 (2019). PubMed: 30590988
- Zhao Z et al. Upregulation of Insulin-Like Growth Factor-1 Receptor (IGF-1R) Reverses the Inhibitory Effect of Let-7g-5p on Migration and Invasion of Nasopharyngeal Carcinoma. Med Sci Monit 25:5747-5756 (2019). PubMed: 31374070
- Wen Z et al. Inhibition of lung cancer cells and Ras/Raf/MEK/ERK signal transduction by ectonucleoside triphosphate phosphohydrolase-7 (ENTPD7). Respir Res 20:194 (2019). PubMed: 31443651
- Zhang Y et al. Sirtuin 6 overexpression relieves sepsis-induced acute kidney injury by promoting autophagy. Cell Cycle 18:425-436 (2019). PubMed: 30700227