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  1. Link

    products/secondary-antibodies/mouse-monoclonal-jdc-10-human-igg-fc-ab99757.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Mouse monoclonal [JDC-10] Anti-Human IgG Fc (ab99757)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (3)

Product price, shipping and contact information

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Mouse monoclonal [JDC-10] Anti-Human IgG Fc
  • Host species: Mouse
  • Isotype: IgG1
  • Suitable for: ICC/IF, ELISA, WB, Dot blot, ICC, IHC-Fr

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Overview

  • Product name

    Mouse monoclonal [JDC-10] Anti-Human IgG Fc
    See all IgG secondary antibodies
  • Host species

    Mouse
  • Target species

    Human
  • Specificity

    ab99757 reacts with the Fc portion of the heavy chain of all subclasses of Human IgG as demonstrated by ELISA; may also react with IgG from other species.
  • Tested applications

    Suitable for: ICC/IF, ELISA, WB, Dot blot, ICC, IHC-Frmore details

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 8.20
    Constituent: 100% Borate buffered saline

    No preservatives or amine-containing buffer salts added.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    JDC-10
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Human
    • IgG
    • Unconjugated

Associated products

  • Alternative Versions

    • Mouse monoclonal [JDC-10] Anti-Human IgG Fc (Alkaline Phosphatase) (ab99758)
    • Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)
    • Mouse monoclonal [JDC-10] Anti-Human IgG heavy chain (Biotin) (ab99760)
    • Mouse monoclonal [JDC-10] Anti-Human IgG Fc (PE) (ab99761)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab99757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use 1µg for 106 cells. for indirect immunofluorescent staining of IgG+ Human B lymphocytes.
ELISA
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.
Dot blot
Use at an assay dependent dilution.
ICC
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Notes
ICC/IF
Use 1µg for 106 cells. for indirect immunofluorescent staining of IgG+ Human B lymphocytes.
ELISA
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.
Dot blot
Use at an assay dependent dilution.
ICC
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab99757? Please let us know so that we can cite the reference in this datasheet.

ab99757 has been referenced in 3 publications.

  • Pedrosa MA  et al. Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19. Clin Sci (Lond) 135:465-481 (2021). PubMed: 33479758
  • Long Y  et al. A non-RBM targeted RBD specific antibody neutralizes SARS-CoV-2 inducing S1 shedding. Biochem Biophys Res Commun 571:152-158 (2021). PubMed: 34325131
  • Chen SL  et al. Targeting CRMP-4 by lentivirus-mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth. Exp Ther Med 12:2003-2008 (2016). PubMed: 27698685

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Hello,

I am curious if any of the mouse anti-human IgG Mabs listed below cross react with monkey IgG?

Mouse anti-Human IgG Fc secondary antibody (ab99757)

Mouse monoclonal [H2] SecondaryAntibody to Human IgG - Fc (ab99770)

Mouse anti-Human IgG1 Fc secondary antibody (ab1927)

Mouse anti-Human IgG heavy chain secondary antibody (ab9243)

Mouse monoclonal [hIgG1] SecondaryAntibody to Human IgG - H&L, pre-adsorbed (ab436)

Read More

Abcam community

Verified customer

Asked on Mar 19 2012

Answer

Thank you for contacting Abcam.

We have not tested any of those antibodies against monkey IgG, therefore I cannot say whether they will cross react or not. Due to this, you can take advantage of our testing discount program if you want, please see the link below for more details:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



If there is anything else I can help you with, please let me know.

Read More

Abcam Scientific Support

Answered on Mar 19 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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