Mouse monoclonal [JDC-10] Anti-Human IgG Fc (ab99757)
Key features and details
- Mouse monoclonal [JDC-10] Anti-Human IgG Fc
- Host species: Mouse
- Isotype: IgG1
- Suitable for: ICC/IF, ELISA, WB, Dot blot, ICC, IHC-Fr
Related conjugates and formulations
Overview
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Product name
Mouse monoclonal [JDC-10] Anti-Human IgG Fc
See all IgG secondary antibodies -
Host species
Mouse -
Target species
Human -
Specificity
ab99757 reacts with the Fc portion of the heavy chain of all subclasses of Human IgG as demonstrated by ELISA; may also react with IgG from other species. -
Tested applications
Suitable for: ICC/IF, ELISA, WB, Dot blot, ICC, IHC-Frmore details
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Constituent: 100% Borate buffered saline
No preservatives or amine-containing buffer salts added. -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
JDC-10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use 1µg for 106 cells. for indirect immunofluorescent staining of IgG+ Human B lymphocytes.
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| ELISA |
Use at an assay dependent dilution.
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| WB |
Use at an assay dependent dilution.
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| Dot blot |
Use at an assay dependent dilution.
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| ICC |
Use at an assay dependent dilution.
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| IHC-Fr |
Use at an assay dependent dilution.
|
| Notes |
|---|
|
ICC/IF
Use 1µg for 106 cells. for indirect immunofluorescent staining of IgG+ Human B lymphocytes. |
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ELISA
Use at an assay dependent dilution. |
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WB
Use at an assay dependent dilution. |
|
Dot blot
Use at an assay dependent dilution. |
|
ICC
Use at an assay dependent dilution. |
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IHC-Fr
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab99757 has been referenced in 7 publications.
- Wang P et al. Precise location of two novel linear epitopes on the receptor-binding domain surface of MERS-CoV spike protein recognized by two different monoclonal antibodies. Int J Biol Macromol 195:609-619 (2022). PubMed: 34871658
- Liang Y et al. Monomeric C-reactive protein level is associated with osteoarthritis. Exp Ther Med 23:277 (2022). PubMed: 35317443
- Pedrosa MA et al. Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19. Clin Sci (Lond) 135:465-481 (2021). PubMed: 33479758
- Long Y et al. A non-RBM targeted RBD specific antibody neutralizes SARS-CoV-2 inducing S1 shedding. Biochem Biophys Res Commun 571:152-158 (2021). PubMed: 34325131
- Phillips DC et al. Hexavalent TRAIL Fusion Protein Eftozanermin Alfa Optimally Clusters Apoptosis-Inducing TRAIL Receptors to Induce On-Target Antitumor Activity in Solid Tumors. Cancer Res 81:3402-3414 (2021). PubMed: 33687950
- Xiong S et al. DNA methyltransferase 3 beta mediates the methylation of the microRNA-34a promoter and enhances chondrocyte viability in osteoarthritis. Bioengineered 12:11138-11155 (2021). PubMed: 34783292
- Chen SL et al. Targeting CRMP-4 by lentivirus-mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth. Exp Ther Med 12:2003-2008 (2016). PubMed: 27698685