Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)
![ELISA - Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)](https://www.abcam.com/ps/products/99/ab99759/Images/ab99759-331754-mouse-monoclonal-jdc-10-anti-human-igg-fc-hrp-elisa.jpg "ELISA - Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)")
Key features and details
- Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP)
- Conjugation: HRP
- Host species: Mouse
- Isotype: IgG1
- Suitable for: ELISA, WB, IHC-Fr, Dot blot, ICC
Related conjugates and formulations
Overview
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Product name
Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP)
See all IgG secondary antibodies -
Host species
Mouse -
Target species
Human -
Specificity
ab99759 reacts with the Fc portion of the heavy chain of all subclasses of Human IgG as demonstrated by ELISA; may also react with IgG from other species. -
Tested applications
Suitable for: ELISA, WB, IHC-Fr, Dot blot, ICCmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol, 50% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
JDC-10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
1/1000 - 1/2000.
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| WB |
Use at an assay dependent dilution.
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| IHC-Fr |
Use at an assay dependent dilution.
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| Dot blot |
Use at an assay dependent dilution.
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| ICC |
Use at an assay dependent dilution.
|
| Notes |
|---|
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ELISA
1/1000 - 1/2000. |
|
WB
Use at an assay dependent dilution. |
|
IHC-Fr
Use at an assay dependent dilution. |
|
Dot blot
Use at an assay dependent dilution. |
|
ICC
Use at an assay dependent dilution. |
Images
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ELISA - Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)
ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted ab99759.
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![ELISA - Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)](https://www.abcam.com/ps/products/99/ab99759/Images/ab99759-331755-mouse-monoclonal-jdc-10-anti-human-igg-fc-hrp-elisa.jpg)
ELISA - Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted ab99759.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab99759 has been referenced in 7 publications.
- Staudacher AH et al. The RNA-binding protein La/SSB associates with radiation-induced DNA double-strand breaks in lung cancer cell lines. Cancer Rep (Hoboken) 5:e1543 (2022). PubMed: 34636174
- Bi J et al. A Novel and Secure Pseudovirus Reporter System Based Assay for Neutralizing and Enhancing Antibody Assay Against Marburg Virus. Front Microbiol 13:927122 (2022). PubMed: 35756049
- Li T et al. Potent SARS-CoV-2 neutralizing antibodies with protective efficacy against newly emerged mutational variants. Nat Commun 12:6304 (2021). PubMed: 34728625
- Alrashoudi AA et al. Fabrication of a Lateral Flow Assay for Rapid In-Field Detection of COVID-19 Antibodies Using Additive Manufacturing Printing Technologies. Int J Bioprint 7:399 (2021). PubMed: 34805593
- Zamora-Gálvez A et al. Photoluminescent lateral flow based on non-radiative energy transfer for protein detection in human serum. Biosens Bioelectron 100:208-213 (2018). PubMed: 28915385
- Horie T et al. Identification of OmpA-Like Protein of Tannerella forsythia as an O-Linked Glycoprotein and Its Binding Capability to Lectins. PLoS One 11:e0163974 (2016). ELISA . PubMed: 27711121
- Akiyama S et al. Prevalence of anti-phospholipase A2 receptor antibodies in Japanese patients with membranous nephropathy. Clin Exp Nephrol 19:653-60 (2015). WB . PubMed: 25412738