Mouse monoclonal [JDC-10] Anti-Human IgG heavy chain (Biotin) (ab99760)
Key features and details
- Mouse monoclonal [JDC-10] Anti-Human IgG heavy chain (Biotin)
- Conjugation: Biotin
- Host species: Mouse
- Isotype: IgG1
- Suitable for: ICC/IF, ELISA, WB, Dot blot, ICC, IHC-Fr
Overview
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Product name
Mouse monoclonal [JDC-10] Anti-Human IgG heavy chain (Biotin)
See all IgG secondary antibodies -
Host species
Mouse -
Target species
Human -
Specificity
ab99760 reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA; may also react with IgG from other species. -
Tested applications
Suitable for: ICC/IF, ELISA, WB, Dot blot, ICC, IHC-Frmore details -
Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
JDC-10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use 1µg for 106 cells.
|
|
| ELISA |
1/1000 - 1/2000.
|
|
| WB |
Use at an assay dependent dilution.
|
|
| Dot blot |
Use at an assay dependent dilution.
|
|
| ICC |
Use at an assay dependent dilution.
|
|
| IHC-Fr |
Use at an assay dependent dilution.
|
| Notes |
|---|
|
ICC/IF
Use 1µg for 106 cells. |
|
ELISA
1/1000 - 1/2000. |
|
WB
Use at an assay dependent dilution. |
|
Dot blot
Use at an assay dependent dilution. |
|
ICC
Use at an assay dependent dilution. |
|
IHC-Fr
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab99760 has not yet been referenced specifically in any publications.