Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
Key features and details
- Rabbit Anti-Chicken IgY H&L (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Suitable for: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, ICC/IF
Related conjugates and formulations
Overview
-
Product name
Rabbit Anti-Chicken IgY H&L (Biotin)
See all IgY secondary antibodies -
Host species
Rabbit -
Target species
Chicken -
Tested applications
Suitable for: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, ICC/IFmore details -
Immunogen
chicken IgY whole molecule
-
Conjugation
Biotin
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule. -
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| WB |
1/2000 - 1/20000.
|
|
| Dot blot |
Use at an assay dependent concentration.
|
|
| ELISA |
1/500000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/1000 - 1/5000.
|
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
WB
1/2000 - 1/20000. |
|
Dot blot
Use at an assay dependent concentration. |
|
ELISA
1/500000. |
|
IHC-P
Use at an assay dependent concentration. |
|
ICC/IF
1/1000 - 1/5000. |
Images
-
Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
-
Immunohistochemistry (Frozen sections) - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (10)
ab6752 has been referenced in 10 publications.
- López-Fernández A et al. Effect of Allogeneic Cell-Based Tissue-Engineered Treatments in a Sheep Osteonecrosis Model. Tissue Eng Part A 26:993-1004 (2020). PubMed: 32122263
- Caminal M et al. A reproducible method for the isolation and expansion of ovine mesenchymal stromal cells from bone marrow for use in regenerative medicine preclinical studies. J Tissue Eng Regen Med 11:3408-3416 (2017). PubMed: 27860364
- McKenzie JA et al. Loss of scleraxis in mice leads to geometric and structural changes in cortical bone, as well as asymmetry in fracture healing. FASEB J 31:882-892 (2017). PubMed: 27864378
- Ren J et al. Imaging rhodopsin degeneration in vivo in a new model of ocular ischemia in living mice. FASEB J 30:612-23 (2016). PubMed: 26443823
- Weis B et al. Inhibition of intestinal tumor formation by deletion of the DNA methyltransferase 3a. Oncogene 0:N/A (2014). PubMed: 24837369
- Jiao TT et al. Importance of spondin 1 and cellular retinoic acid binding protein 1 in the clinical diagnosis of ovarian cancer. Int J Clin Exp Pathol 6:3036-41 (2013). IHC-P . PubMed: 24294399
- Gakis G et al. XPA-210: a new proliferation marker to characterize tumor biology and progression of renal cell carcinoma. World J Urol 29:801-6 (2011). IHC-P . PubMed: 21113600
- Wong EW et al. Regulation of blood-testis barrier dynamics by TGF-beta3 is a Cdc42-dependent protein trafficking event. Proc Natl Acad Sci U S A 107:11399-404 (2010). IHC-Fr . PubMed: 20534521
- Koryakina YA et al. Characterization of a panel of six beta2-adrenergic receptor antibodies by indirect immunofluorescence microscopy. Respir Res 9:32 (2008). ICC/IF . PubMed: 18423037
- Ergün S et al. Cell type-specific expression of LINE-1 open reading frames 1 and 2 in fetal and adult human tissues. J Biol Chem 279:27753-63 (2004). PubMed: 15056671