Rabbit Anti-Chicken IgY H&L (FITC) (ab6749)
Key features and details
- Rabbit Anti-Chicken IgY H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Rabbit
- Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Chicken IgY H&L (FITC)
See all IgY secondary antibodies -
Host species
Rabbit -
Target species
Chicken -
Tested applications
Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details -
Immunogen
chicken IgY whole molecule
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Immunomicroscopy |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent dilution.
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| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
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| ICC/IF |
1/1000 - 1/5000. PubMed: 18983843
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| ELISA |
1/10000 - 1/50000.
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| Notes |
|---|
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Immunomicroscopy
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent dilution. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
1/1000 - 1/5000. PubMed: 18983843 |
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ELISA
1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab6749 has been referenced in 12 publications.
- de Graaf JF et al. Optimizing environmental safety and cell-killing potential of oncolytic Newcastle Disease virus with modifications of the V, F and HN genes. PLoS One 17:e0263707 (2022). PubMed: 35139115
- Cheng H et al. Cyclic Strain and Electrical Co-stimulation Improve Neural Differentiation of Marrow-Derived Mesenchymal Stem Cells. Front Cell Dev Biol 9:624755 (2021). PubMed: 34055769
- Kiese K et al. Epigenetic control of epilepsy target genes contributes to a cellular memory of epileptogenesis in cultured rat hippocampal neurons. Acta Neuropathol Commun 5:79 (2017). PubMed: 29089052
- Cheng YC et al. Albumin overload down-regulates integrin-ß1 through reactive oxygen species-endoplasmic reticulum stress pathway in podocytes. J Biochem 158:101-8 (2015). PubMed: 25713411
- Buijs PR et al. Different responses of human pancreatic adenocarcinoma cell lines to oncolytic Newcastle disease virus infection. Cancer Gene Ther 21:24-30 (2014). PubMed: 24384773