Rabbit Anti-Chicken IgY H&L (HRP) (ab6753)
Key features and details
- Rabbit Anti-Chicken IgY H&L (HRP)
- Conjugation: HRP
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Chicken IgY H&L (HRP)
See all IgY secondary antibodies -
Host species
Rabbit -
Target species
Chicken -
Tested applications
Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details -
Immunogen
chicken IgY whole molecule
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Do not add Sodium Azide -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Horseradish Peroxidase (HRP). -
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
Clonality
Polyclonal -
Isotype
IgG -
General notes
May cross-react with chicken IgM
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Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000 - 1/5000.
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Dot blot |
Use at an assay dependent concentration.
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ELISA |
1/50000 - 1/100000.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Immunomicroscopy |
Use at an assay dependent concentration.
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|
WB |
1/2500 - 1/10000.
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Notes |
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ICC/IF
1/1000 - 1/5000. |
Dot blot
Use at an assay dependent concentration. |
ELISA
1/50000 - 1/100000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Immunomicroscopy
Use at an assay dependent concentration. |
WB
1/2500 - 1/10000. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab6753 has been referenced in 26 publications.
- Castellanos-Huerta I et al. Transformation of Dunaliella salina by Agrobacterium tumefaciens for the Expression of the Hemagglutinin of Avian Influenza Virus H5. Microorganisms 10:N/A (2022). PubMed: 35208815
- Schmerl B et al. The synaptic scaffold protein MPP2 interacts with GABAA receptors at the periphery of the postsynaptic density of glutamatergic synapses. PLoS Biol 20:e3001503 (2022). PubMed: 35312684
- Wang Y et al. Delivering Antisense Oligonucleotides across the Blood-Brain Barrier by Tumor Cell-Derived Small Apoptotic Bodies. Adv Sci (Weinh) 8:2004929 (2021). PubMed: 34258157
- Lee W et al. MeCP2 regulates gene expression through recognition of H3K27me3. Nat Commun 11:3140 (2020). PubMed: 32561780
- Abbas AT et al. Anti-S1 MERS-COV IgY Specific Antibodies Decreases Lung Inflammation and Viral Antigen Positive Cells in the Human Transgenic Mouse Model. Vaccines (Basel) 8:N/A (2020). PubMed: 33139631