Rabbit F(ab')2 Anti-Dog IgG H&L (Alkaline Phosphatase) (ab136764)
Key features and details
- Rabbit F(ab')2 Anti-Dog IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-Fr, IHC-P, Functional Studies, ELISA, WB, ICC
Related conjugates and formulations
Overview
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Product name
Rabbit F(ab')2 Anti-Dog IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Dog -
Tested applications
Suitable for: IHC-Fr, IHC-P, Functional Studies, ELISA, WB, ICCmore details -
Immunogen
Highly purified whole immunoglobulin from dog
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 48.9% Tris buffered saline, 50% Glycerol, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab136764 is purified by affinity chromatography and is thus particularly specific. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Preparation of Substrate Solutions:
ELISA : 1M diethanolamin, 0.5mM MgCl2, 15mM pNPP, pH 9.8. Incubate for 15 minutes at 37°C. Stop the reaction with V/5 K2HP04 2M. Read at 405 nm.
Immunoblot : 34 µl of BCIP (5bromo-4chloroindolylphosphate) at 50mg/ml in DMF + 44 µl NBT (NitroBlue Tetrazolium) at 75 mg/ml in DMF 70% + 10ml substrate buffer (0.1M TRIS/HCI, 0.5M NaCl, 0.05M MgCl2, pH 9.5). -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
1/25 - 1/100.
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IHC-P |
1/25 - 1/100.
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Functional Studies |
Use at an assay dependent concentration.
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ELISA |
1/500 - 1/5000.
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WB |
1/100 - 1/1000.
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ICC |
1/25 - 1/100.
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Notes |
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IHC-Fr
1/25 - 1/100. |
IHC-P
1/25 - 1/100. |
Functional Studies
Use at an assay dependent concentration. |
ELISA
1/500 - 1/5000. |
WB
1/100 - 1/1000. |
ICC
1/25 - 1/100. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab136764 has not yet been referenced specifically in any publications.