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Rabbit F(ab')2 Anti-Dog IgG H&L (Biotin) (ab136769)

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Key features and details

  • Rabbit F(ab')2 Anti-Dog IgG H&L (Biotin)
  • Conjugation: Biotin
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: Flow Cyt, IHC-Fr, IHC-P, ICC, WB, ELISA

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Rabbit F(ab')2 Anti-Dog IgG H&L (Biotin)
    See all IgG secondary antibodies

  • Host species

    Rabbit

  • Target species

    Dog

  • Tested applications

    Suitable for: Flow Cyt, IHC-Fr, IHC-P, ICC, WB, ELISAmore details

  • Immunogen

    Highly purified whole immunoglobulin from Dog.

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituents: 50% PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab136769 is purified by affinity chromatography and is thus particularly specific.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab136769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (1)
Use at an assay dependent concentration.
IHC-Fr
1/50 - 1/200.
IHC-P
1/50 - 1/200.
ICC
1/50 - 1/200.
WB
1/200 - 1/2000.
ELISA
1/2000 - 1/20000.
Notes
Flow Cyt
Use at an assay dependent concentration.
IHC-Fr
1/50 - 1/200.
IHC-P
1/50 - 1/200.
ICC
1/50 - 1/200.
WB
1/200 - 1/2000.
ELISA
1/2000 - 1/20000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab136769? Please let us know so that we can cite the reference in this datasheet.

ab136769 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Magpix assay

 Good
Abreviews
abreview image
Application
Flow Cytometry
The sample used was dog serum. The beads coated with peptides were combined with dog reference serum. Then incubated in the dark on a shaker for 30 mins. They were washed and secondary antibody Rb anti dog IgG H+L was added. This was allowed to incubate as well under similar conditions. Then detection antibody Strepavadin PE was added and detected on the MagPix.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Anushka George

Verified customer

Submitted Mar 16 2016

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