Rabbit F(ab')2 Anti-Mouse IgG - H&L (TR), pre-adsorbed (ab5759)
Key features and details
- Rabbit F(ab')2 Anti-Mouse IgG - H&L (TR), pre-adsorbed
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISA
Related conjugates and formulations
Overview
-
Product name
Rabbit F(ab')2 Anti-Mouse IgG - H&L (TR), pre-adsorbed
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Mouse -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins. -
Tested applications
Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details -
Minimal
cross-reactivity
Human more details -
Immunogen
Mouse IgG whole molecule
-
Conjugation
Texas Red ®. Ex: 596nm, Em: 620nm
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
Conjugation notes
Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.3 moles Texas Red per mole of Rabbit IgG F(ab’)2 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
|
|
| Immunomicroscopy |
Use at an assay dependent dilution.
|
|
| Flow Cyt |
1/500 - 1/2500.
|
|
| ELISA |
1/10000 - 1/50000.
|
| Notes |
|---|
|
ICC/IF
1/1000 - 1/5000. |
|
Immunomicroscopy
Use at an assay dependent dilution. |
|
Flow Cyt
1/500 - 1/2500. |
|
ELISA
1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (1)
ab5759 has been referenced in 1 publication.
- Lian S et al. PRL-3 promotes telomere deprotection and chromosomal instability. Nucleic Acids Res 45:6546-6571 (2017). PubMed: 28482095
