Rabbit Anti-Goat IgG H&L (beta-galactosidase) (ab136712)
Key features and details
- Rabbit Anti-Goat IgG H&L (beta-galactosidase)
- Conjugation: beta-galactosidase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-Fr, IHC-P, ICC, WB, ELISA
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Goat IgG H&L (beta-galactosidase)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Goat -
Tested applications
Suitable for: IHC-Fr, IHC-P, ICC, WB, ELISAmore details -
Immunogen
Highly purified whole immunoglobulin from Goat.
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Conjugation
beta-galactosidase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab136712 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography). -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Preparation of Substrate Solutions: 0.1M phosphate buffer pH7, 1mM MgSO4, 0.2mM MnSO4, 2mM Mg titriplex. Add extemporaneously 7µl/ml beta-mercaptoethanol and 0.9mg/ml ortho-nitrophenyl-beta-galactopyranoside. Incubate for 30 minutes at 37°C. Stop the reaction with V/2 volume of 1M Na2CO3. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| ICC |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration.
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| ELISA |
1/100 - 1/1000.
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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ICC
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
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ELISA
1/100 - 1/1000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab136712 has been referenced in 3 publications.
- Qiao D et al. Paramyxovirus replication induces the hexosamine biosynthetic pathway and mesenchymal transition via the IRE1α-XBP1s arm of the unfolded protein response. Am J Physiol Lung Cell Mol Physiol 321:L576-L594 (2021). PubMed: 34318710
- Bilawchuk LM et al. The Susceptibilities of Respiratory Syncytial Virus to Nucleolin Receptor Blocking and Antibody Neutralization are Dependent upon the Method of Virus Purification. Viruses 9:N/A (2017). PubMed: 28771197
- Elawar F et al. A Virological and Phylogenetic Analysis of the Emergence of New Clades of Respiratory Syncytial Virus. Sci Rep 7:12232 (2017). PubMed: 28947776