Rabbit Anti-Goat IgG H&L (HRP) preadsorbed (ab7132)
Key features and details
- Rabbit Anti-Goat IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Rabbit
- Suitable for: ELISA, IHC-P, IHC-Fr, WB, ICC
Overview
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Product name
Rabbit Anti-Goat IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Goat -
Specificity
Pre-adsorbed against Human Serum Proteins to minimise reactivity. -
Tested applications
Suitable for: ELISA, IHC-P, IHC-Fr, WB, ICCmore details -
Minimal
cross-reactivity
Human more details -
Immunogen
Goat IgG whole molecule
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Horseradish Peroxidase (HRP) -
Clonality
Polyclonal -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
1/20000 - 1/120000.
|
|
| IHC-P |
1/500 - 1/2500.
|
|
| IHC-Fr |
1/500 - 1/2500.
|
|
| WB |
1/1000 - 1/5000.
|
|
| ICC |
1/500 - 1/2500.
|
| Notes |
|---|
|
ELISA
1/20000 - 1/120000. |
|
IHC-P
1/500 - 1/2500. |
|
IHC-Fr
1/500 - 1/2500. |
|
WB
1/1000 - 1/5000. |
|
ICC
1/500 - 1/2500. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab7132 has been referenced in 6 publications.
- Moberg M et al. High-intensity leg cycling alters the molecular response to resistance exercise in the arm muscles. Sci Rep 11:6453 (2021). PubMed: 33742064
- Issa AR et al. The lysosomal membrane protein LAMP2A promotes autophagic flux and prevents SNCA-induced Parkinson disease-like symptoms in the Drosophila brain. Autophagy 14:1898-1910 (2018). PubMed: 29989488
- Rundqvist HC et al. Influence of nutrient ingestion on amino acid transporters and protein synthesis in human skeletal muscle after sprint exercise. J Appl Physiol (1985) 123:1501-1515 (2017). PubMed: 28860165
- Moberg M et al. Activation of mTORC1 by leucine is potentiated by branched-chain amino acids and even more so by essential amino acids following resistance exercise. Am J Physiol Cell Physiol 310:C874-84 (2016). PubMed: 27053525
- Apró W et al. Resistance exercise-induced S6K1 kinase activity is not inhibited in human skeletal muscle despite prior activation of AMPK by high-intensity interval cycling. Am J Physiol Endocrinol Metab 308:E470-81 (2015). PubMed: 25605643
- Kassner N et al. CBR1 is a predominant doxorubicin reductase in the human liver. Drug Metab Dispos : (2008). WB . PubMed: 18635746