Rabbit Anti-Human IgG H&L (Texas Red ®) (ab6757)
Key features and details
- Rabbit Anti-Human IgG H&L (Texas Red ®)
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Host species: Rabbit
- Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ChIP, ELISA
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Human IgG H&L (Texas Red ®)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Human -
Tested applications
Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ChIP, ELISAmore details -
Immunogen
human IgG whole molecule
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Conjugation
Texas Red ®. Ex: 596nm, Em: 620nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG -
Clonality
Polyclonal -
Research areas
Associated products
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ChIP Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Immunomicroscopy |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
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| ICC/IF |
1/1000 - 1/5000.
|
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| IHC-P |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| ChIP |
Use a concentration of 6 µg/ml. PubMed: 17355974
|
|
| ELISA |
1/10000 - 1/50000.
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| Notes |
|---|
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Immunomicroscopy
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. |
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ICC/IF
1/1000 - 1/5000. |
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IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ChIP
Use a concentration of 6 µg/ml. PubMed: 17355974 |
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ELISA
1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab6757 has been referenced in 3 publications.
- Liang Y et al. Superenhancer-transcription factor regulatory network in malignant tumors. Open Med (Wars) 16:1564-1582 (2021). PubMed: 34722892
- Li JQ et al. Generation of induced pluripotent stem cells using skin fibroblasts from patients with myocardial infarction under feeder-free conditions. Mol Med Rep 9:837-42 (2014). PubMed: 24398533
- Makino Y et al. Transcriptional up-regulation of inhibitory PAS domain protein gene expression by hypoxia-inducible factor 1 (HIF-1): a negative feedback regulatory circuit in HIF-1-mediated signaling in hypoxic cells. J Biol Chem 282:14073-82 (2007). PubMed: 17355974