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  1. Link

    products/secondary-antibodies/rabbit-human-igg-hl-texas-red--ab6757.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Rabbit Anti-Human IgG H&L (Texas Red ®) (ab6757)

  • Datasheet
  • SDS
Submit a review Submit a question References (3)

Product price, shipping and contact information

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Promotion Information

Abpromise

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Find out more.

Key features and details

  • Rabbit Anti-Human IgG H&L (Texas Red ®)
  • Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
  • Host species: Rabbit
  • Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ChIP, ELISA

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Overview

  • Product name

    Rabbit Anti-Human IgG H&L (Texas Red ®)
    See all IgG secondary antibodies
  • Host species

    Rabbit
  • Target species

    Human
  • Tested applications

    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ChIP, ELISAmore details
  • Immunogen

    human IgG whole molecule

  • Conjugation

    Texas Red ®. Ex: 596nm, Em: 620nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG
  • Clonality

    Polyclonal
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Human
    • IgG
    • Fluorophore
    • Texas Red

Associated products

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ChIP
Use a concentration of 6 µg/ml. PubMed: 17355974
ELISA
1/10000 - 1/50000.
Notes
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ChIP
Use a concentration of 6 µg/ml. PubMed: 17355974
ELISA
1/10000 - 1/50000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab6757? Please let us know so that we can cite the reference in this datasheet.

ab6757 has been referenced in 3 publications.

  • Liang Y  et al. Superenhancer-transcription factor regulatory network in malignant tumors. Open Med (Wars) 16:1564-1582 (2021). PubMed: 34722892
  • Li JQ  et al. Generation of induced pluripotent stem cells using skin fibroblasts from patients with myocardial infarction under feeder-free conditions. Mol Med Rep 9:837-42 (2014). PubMed: 24398533
  • Makino Y  et al. Transcriptional up-regulation of inhibitory PAS domain protein gene expression by hypoxia-inducible factor 1 (HIF-1): a negative feedback regulatory circuit in HIF-1-mediated signaling in hypoxic cells. J Biol Chem 282:14073-82 (2007). PubMed: 17355974

Customer reviews and Q&As

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