Recombinant Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc (ab125909)
![ELISA - Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc (ab125909)](https://www.abcam.com/ps/products/125/ab125909/Images/ab125909-366347-rabbit-monoclonal-h169-1-5-anti-human-igg-fc-elisa-human-monkey-igg.jpg "ELISA - Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc (ab125909)")
Key features and details
- Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ELISA
Related conjugates and formulations
Overview
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Product name
Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Human -
Tested applications
Suitable for: ELISAmore details -
Immunogen
The details of the immunogen for this antibody are not available.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
H169-1-5 -
Isotype
IgG -
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
1/1000.
|
| Notes |
|---|
|
ELISA
1/1000. |
Images
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ELISA - Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc (ab125909)
Direct ELISA antibody dose-response curve using ab125909 between 0-1000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
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![ELISA - Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc (ab125909)](https://www.abcam.com/ps/products/125/ab125909/Images/ab125909-289012-rabbit-monoclonal-h169-1-5-anti-human-igg-fc-elisa.jpg)
ELISA - Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc (ab125909)Direct ELISA antibody dose-response curve using ab125909 between 0-1000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
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![ELISA - Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc (ab125909)](https://www.abcam.com/ps/products/125/ab125909/Images/ab125909-289013-rabbit-monoclonal-h169-1-5-anti-human-igg-fc-elisa.jpg)
ELISA - Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc (ab125909)Direct ELISA antibody dose-response curve using ab125909 between 0-1000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab125909 has been referenced in 2 publications.
- Kostov K & Blazhev A Serum Anti-Collagen IV IgM and IgG Antibodies as Indicators of Low Vascular Turnover of Collagen IV in Patients with Long-Term Complications of Type 2 Diabetes. Diagnostics (Basel) 11:N/A (2021). PubMed: 34069322
- Puligedda RD et al. Capture and display of antibodies secreted by hybridoma cells enables fluorescent on-cell screening. MAbs 11:546-558 (2019). PubMed: 30794061