Recombinant Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc - BSA and Azide free (ab288280)
Key features and details
- Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc - BSA and Azide free
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ELISA
Related conjugates and formulations
Overview
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Product name
Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc - BSA and Azide free
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Human -
Specificity
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Tested applications
Suitable for: ELISAmore details -
Immunogen
The details of the immunogen for this antibody are not available.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
H169-1-5 -
Isotype
IgG -
General notes
ab288280 is the carrier-free version of ab125909
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab288280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
Images
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This data was developed using ab125909, the same antibody clone in a different buffer formulation.
Direct ELISA antibody dose-response curve using ab125909 between 0-1000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
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This data was developed using ab125909, the same antibody clone in a different buffer formulation.
Direct ELISA antibody dose-response curve using ab125909 between 0-1000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
-
This data was developed using ab125909, the same antibody clone in a different buffer formulation.
Direct ELISA antibody dose-response curve using ab125909 between 0-1000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab288280 has not yet been referenced specifically in any publications.