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RecombinantRabMAb

Recombinant Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)

Submit a review Submit a question References (1)
Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)
  • Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)
  • ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)
  • ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)
  • Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)
  • ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)

Key features and details

  • Rabbit monoclonal [H19-5] Anti-Human kappa light chain
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: WB, ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Biotin Carrier Free

Overview

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 99% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    H19-5

  • Isotype

    IgG

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab125919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000.

For unpurified use at 1/1000 - 1/10000.
ELISA
Use a concentration of 1 µg/ml.

For unpurified use at 1/1000 - 1/10000.
Notes
WB
1/5000.

For unpurified use at 1/1000 - 1/10000.
ELISA
Use a concentration of 1 µg/ml.

For unpurified use at 1/1000 - 1/10000.

Images

  • Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)
    Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)
    All lanes : Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919) at 1/5000 dilution

    Lane 1 : Human spleen tissue lysate
    Lane 2 : Human thymus tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Observed band size: 25 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)
    Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)
    Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919) at 1/5000 dilution (purified) + Human tonsil tissue lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Observed band size: 25 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)
    ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)

    Direct ELISA antibody dose-response curve using ab125919 between 0-2500 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)
    ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)

    Direct ELISA antibody dose-response curve using ab125919 between 0-2500 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)
    Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)
    All lanes : Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919) (unpurified)

    Lane 1 : Human spleen tissue lysate
    Lane 2 : Human thymus tissue lysate
    Lane 3 : Human tonsil tissue lysate
  • ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)
    ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)

    ELISA dose-responsive curve using purified ab125919 at a range of concentrations.

    Antigen concentation: 1000ng/ml.

    Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), 1/2500.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab125919? Please let us know so that we can cite the reference in this datasheet.

ab125919 has been referenced in 1 publication.

  • Seo Y  et al. Assembly and Folding Properties of Cytosolic IgG Intrabodies. Sci Rep 10:2140 (2020). PubMed: 32034177

Customer reviews and Q&As

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