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RecombinantRabMAb

Recombinant Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (ab125912)

Submit a review Q&A (2)
ELISA - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (ab125912)
  • ELISA - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (ab125912)

Key features and details

  • Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Biotin Carrier Free

Overview

  • Product name

    Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L
    See all IgG1 secondary antibodies

  • Host species

    Rabbit

  • Target species

    Human

  • Tested applications

    Suitable for: ELISAmore details

  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    H26-10

  • Isotype

    IgG

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab125912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/1000.
Notes
ELISA
1/1000.

Images

  • ELISA - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (ab125912)
    ELISA - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (ab125912)

    Direct ELISA antibody dose-response curve using ab125912 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • ELISA - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (ab125912)
    ELISA - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (ab125912)

    Direct ELISA antibody dose-response curve using ab125912 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab125912? Please let us know so that we can cite the reference in this datasheet.

ab125912 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

I am interested in Rabbit monoclonal H26-10 Anti-Human IgG1 H&L (ab125912). Do you have a HRP-labeled conjugate of this antibody ? Do you have another recommendation for an anti-human IgG antibody with high affinity (we need highest sensitivity in an ELISA therefore affinity is most important for us).

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Answer


I am sorry to confirm that we do not have an HRP conjugated version of the clone available.

There is the following alternative in the catalog which is tested in ELISA:


Mouse monoclonal 4E3 Anti-Human IgG1 hinge heavy chain (HRP) (ab99774)
https://www.abcam.com/Mouse-monoclonal-4E3-Human-IgG1-hinge-heavy-chain-HRP-ab99774.html (or use the following: https://www.abcam.com/Mouse-monoclonal-4E3-Human-IgG1-hinge-heavy-chain-HRP-ab99774.html).

Or you may like to consider our conjugation kits to conjugate ab125912 with HRP:

HRP Conjugation Kit (ab102890)
https://www.abcam.com/HRP-Conjugation-Kit-Lightning-Linkreg-ab102890.html (or use the following: https://www.abcam.com/HRP-Conjugation-Kit-Lightning-Linkreg-ab102890.html).

When using the conjugation kits I can recommend to review the frequently asked questions page for more information regardign compatability etc.

https://www.abcam.com/index.html?pageconfig=resource&rid=13156


We also have the following ELISA kit which may be useful to consider:

Human IgG ELISA Kit SimpleStep (ab195215)
Description: Human IgG ELISA Kit SimpleStep
Reactivity: Human
Sample type: Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Tissue Lysate

https://www.abcam.com/Human-IgG-ELISA-Kit-ab195215.html (or use the following: https://www.abcam.com/Human-IgG-ELISA-Kit-ab195215.html).

The sensitivity of this kit is Sensitivity 0.02 ng/ml

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Question

Have you determined the dissociation constant of this antibody? Even an estimate would be helpful.

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Answer

Thank you for your inquiry.
The lab has not determined the dissociation constant for this antibody, however rabbit monoclonals typically have very high affinities (typically in the picomolar level).
Minimum: 10^-9 - 10^-10 KDM
Maximum: 10^-12 KDM
I hope this information helps. Please let me know if you have any further questions.

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