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Rabbit Anti-Mouse IgG H&L (ab46540)

Submit a review Q&A (5)References (297)

Key features and details

  • Rabbit Anti-Mouse IgG H&L
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: ELISA, WB

Overview

  • Product name

    Rabbit Anti-Mouse IgG H&L

  • Host species

    Rabbit

  • Target species

    Mouse

  • Specificity

    Recognizes mouse IgG heavy and light chains.

  • Tested applications

    Suitable for: ELISA, WBmore details

  • Immunogen

    Native, mouse whole IgG.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This product was purified by affinity chromatography using mouse IgG-agarose.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab46540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/20000 - 1/100000.
WB
1/2000 - 1/10000.

This product is suitable for IPPT, Immunodiffusion and most immunological methods requiring lot-to-lot consistency, high titre and specificity.
Notes
ELISA
1/20000 - 1/100000.
WB
1/2000 - 1/10000.

This product is suitable for IPPT, Immunodiffusion and most immunological methods requiring lot-to-lot consistency, high titre and specificity.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (297)

Publishing research using ab46540? Please let us know so that we can cite the reference in this datasheet.

ab46540 has been referenced in 297 publications.

  • Sidorenko E  et al. Lamina-associated polypeptide 2a is required for intranuclear MRTF-A activity. Sci Rep 12:2306 (2022). PubMed: 35145145
  • Oh GS  et al. Regulation of Hepatic Gluconeogenesis by Nuclear Receptor Coactivator 6. Mol Cells 45:180-192 (2022). PubMed: 35258009
  • Xiong J  et al. Hypoxia Enhances HIF1a Transcription Activity by Upregulating KDM4A and Mediating H3K9me3, Thus Inducing Ferroptosis Resistance in Cervical Cancer Cells. Stem Cells Int 2022:1608806 (2022). PubMed: 35287356
  • Layden HM  et al. A protocol for rapid degradation of endogenous transcription factors in mammalian cells and identification of direct regulatory targets. STAR Protoc 2:100530 (2021). PubMed: 34041503
  • Singh S  et al. The PfAP2-G2 transcription factor is a critical regulator of gametocyte maturation. Mol Microbiol 115:1005-1024 (2021). PubMed: 33368818
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Question

I need some help designing my ChIP experiment.

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Answer

Thank you for contacting us.


First, I'd like to point out some resources on our website which may help:


https://www.abcam.com/index.html?rid=310


A Beginner's Guide to ChIP - Abcam


As we discussed over the phone, you may want to start with our ChIP kit (ab500)since this has everything you need for the reaction, including the positive control antibody ab1791.


https://www.abcam.com/ChIP-Kit-ab500.html


In addition, you'll need the two antibodies you were interested in, and their matching negative controls.


Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)


https://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-ChIP-Grade-ab8580.html


Rabbit Control IgG - ChIP Grade (ab46540)


https://www.abcam.com/Rabbit-Control-IgG-ChIP-Grade-ab46540.html


Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002)


https://www.abcam.com/Histone-H3-tri-methyl-K27-antibody-mAbcam-6002-ChIP-Grade-ab6002.html


Mouse IgG3, kappa monoclonal [MG3-35] - Isotype control (ab18394). This one isn't tested in ChIP, but I don't see a reason why it wouldn't work.


https://www.abcam.com/Mouse-IgG3-kappa-monoclonal-MG3-35-Isotype-control-ab18394.html


You'll also need the housekeeping primers, like GAPDH, ab83588 and ab83589.

https://www.abcam.com/hsgapdh-chip-primer-exon-1-forward-ab83588.html

https://www.abcam.com/hsgapdh-chip-primer-exon-1-reverse-ab83589.html


That should be enough to get you started, but take a look at the resources mentioned above and let me know if you have any additional questions.

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Question

phone call about rabbit IGG controls for ChIP

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Answer

Merci beaucoup pour votre appel de ce matin.

Je suis désolée de devoir vous confirmer que nous devons corriger et informer les clients du produit ab46540, car, grâce à vous, j'ai vérifié et j'ai trouvéquele ab46540 est effectivement réactif contre les immunoglobulines de souris. Nous allons corriger ceci. Merci d'avoir attiré notre attention à ce fait.

Même si vous travaillez avec du tissu humain, je ne préfère pas vous recommander le ab46540. Le ab37415 est aussi un control pour les IgG de lapin et il était aussi testé en ChIP. Je peux donc vous recommander celui- ci. J'ai aussi double vérifié que le ab37415 ne contient effectivement aucune réactivité connue.

J'espère que ces informations vous sont utiles. Je m'excuse pour ces confusions et nous restons à votre disposition pour tout renseignement supplémentaire.

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Question

Hello,

I'm using Rabbit Control IgG - ChIP Grade (ab46540)
for my chip experiment.
Do you have equivalent for rat and mouse? I don't find them,


Thanks in advance,

Read More
Answer

Thank you for your inquiry.

Indeed we do have also polyclonal control fractions of mouse or rat IgGs.

For the mouse IgG, we do have the IgG fraction ab37355 https://www.abcam.com/Mouse-IgG-Isotype-Control-ab37355.html (or use the following: https://www.abcam.com/Mouse-IgG-Isotype-Control-ab37355.html).

For the rat IgG, we do have the IgG fraction ab37361 https://www.abcam.com/Rat-IgG-Isotype-Control-ab37361.html (or use the following: https://www.abcam.com/Rat-IgG-Isotype-Control-ab37361.html).

These two products have been used as controls so far only in ELISA and flow cytometry to our knowledge. As they have however no known specificity, I would expect them to work also in ChIP. If you would like to test them in ChIP, please do let me know, as I might can provide you with a testing offer.

Otherwise, if you are looking for a monoclonal rat or mouse antibody as a control, we do have also monoclonal isotype controls. Voici la liste de nos anticorps isotypes controls: https://www.abcam.com/index.html?pageconfig=catalog_byclass&intClassID=718&strStartChar=A,B,C,D,E,F,G,H,I,J,K,L,M

Please do not hesitate to contact us again if you have anyquestion, if wecould help you in the further selection of an antibody or if you are interested in testing the above mentioned antibodies in ChIP.

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Question

dear correspondent,

We have lately purchased several Abs from Abcam and i have a question related to the purification method.
In the datasheets of several monoclonal antibodies is mentioned that they are purified as 'IgG fraction'. Could you exlain to me what that means? Is a anti-IgG column used or an protein A/G column (or any other technique)? This is very important information to us, as we need to consider protein A/G contamination to explain data obtained in our R&D dept. in experiments using these antibodies.

More specifically, the question relates to the following antibodies (data sheets of all those abs mention 'IgG fraction' in 'purity' annotation):

ab3609-100 (anti-Aurora B)
ab51841-100 (anti-TATA binding protein)
ab37373 (goat control IgG)
ab46540 (rabbit control IgG)


Thanks in advance and hope to hear your answer soon,

Read More
Answer

Thank you for your enquiry.

I have confirmed the purification details of the antibodies you listed and have updated the datasheets where necessary:

ab3609 (Aurora B)
Protein G purified

ab51841 (TATA binding protein)
Protein G purified

ab37373 (goat control IgG)
Proprietary combination of ammonium sulfate precipitation, ion exchange, and/or other chromatography techniques.

ab46540 (rabbit control IgG)
Affinity purified against the antigen.

I hope this will be helpful. If you require any further information on the purification of other products, or any other questions, please do not hesitate to contact me.

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Question

Dear, We lately bought the rabbit control IgG antibody. We would like to rebuy it but need further information on the following question. Is this antibody purified via protein A chromatography or not? This is important information for us if we want to assess the quality of the ab. This is not clearly mentioned on the datasheet. If you dont know, please also indicate so, so that we can consider protein A contamination in the sample to explain data generated by us using this ab. Thank you very much,

Read More
Answer

Thank you for your inquiry.
I can confim that the antibody was affinity-purified against the antigen itself (bound to agarose).
Protein A or G was not used.
I hope this information is helpful. please do not hesitate to contact me with any further questions.

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