Rabbit Anti-Mouse IgG H&L (ab6709)
Key features and details
- Rabbit Anti-Mouse IgG H&L
- Host species: Rabbit
- Suitable for: WB, IP, Immunodiffusion, Conjugation, ICC/IF, ELISA, IHC-P, IHC-Fr
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Mouse IgG H&L
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Mouse -
Tested applications
Suitable for: WB, IP, Immunodiffusion, Conjugation, ICC/IF, ELISA, IHC-P, IHC-Frmore details -
Immunogen
Mouse IgG, whole molecule
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000.
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| IP |
Use at an assay dependent concentration.
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| Immunodiffusion |
Use at an assay dependent concentration.
|
|
| Conjugation |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/1000 - 1/5000.
|
|
| ELISA |
1/20000 - 1/400000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
1/2000 - 1/10000. |
|
IP
Use at an assay dependent concentration. |
|
Immunodiffusion
Use at an assay dependent concentration. |
|
Conjugation
Use at an assay dependent concentration. |
|
ICC/IF
1/1000 - 1/5000. |
|
ELISA
1/20000 - 1/400000. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
Images
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ELISA - Rabbit Anti-Mouse IgG H&L (ab6709)
Sensitivity of ab6709 against mouse IgG.
The starting dilution of ab6709 was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. The blocking agent was 3% fish gelatin. The detection antibody was a Goat anti-Rabbit IgG Antibody Peroxidase conjugate. TMB color development.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (45)
ab6709 has been referenced in 45 publications.
- Garcia-Martin R et al. MicroRNA sequence codes for small extracellular vesicle release and cellular retention. Nature 601:446-451 (2022). PubMed: 34937935
- Weiss JM et al. Anatomic position determines oncogenic specificity in melanoma. Nature 604:354-361 (2022). PubMed: 35355015
- Ruan Z et al. Functional genome-wide short hairpin RNA library screening identifies key molecules for extracellular vesicle secretion from microglia. Cell Rep 39:110791 (2022). PubMed: 35545052
- Kraft K et al. Polycomb-mediated genome architecture enables long-range spreading of H3K27 methylation. Proc Natl Acad Sci U S A 119:e2201883119 (2022). PubMed: 35617427
- Kitamura K et al. Dual function of SF3B2 on chromatin and RNA to regulate transcription in head and neck squamous cell carcinoma. Cell Biosci 12:92 (2022). PubMed: 35715826