Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150125)
Key features and details
- Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-Fr, ICC/IF, ELISA, Flow Cyt, IHC-P
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG was detected. This antibody may cross react with IgG from other species. -
Tested applications
Suitable for: IHC-Fr, ICC/IF, ELISA, Flow Cyt, IHC-Pmore details -
Minimal
cross-reactivity
Human, Rat more details -
Immunogen
The details of the immunogen for this antibody are not available.
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Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| ICC/IF |
1/200 - 1/1000.
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| ELISA |
Use at an assay dependent concentration.
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| Flow Cyt |
1/2000.
ab170190 - Mouse monoclonal IgG1 (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody.
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| IHC-P |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
1/200 - 1/1000. |
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ELISA
Use at an assay dependent concentration. |
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Flow Cyt
1/2000. ab170190 - Mouse monoclonal IgG1 (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody.
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IHC-P
Use at an assay dependent concentration. |
Images
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Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150125)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab150125 Alexa Fluor® 488 rabbit anti-mouse IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150125)HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (green) was ab150125 Alexa Fluor® 488 rabbit anti-mouse IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Alexa Fluor® - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150125)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab150125 has been referenced in 8 publications.
- Li X et al. Loss-of-function Mutations K11E or E271K Lead to Novel Tumor Suppression, Implicate Nucleolar Helicase DDX24 Oncogenicity. Int J Med Sci 19:596-608 (2022). PubMed: 35370459
- Sultanova A et al. HHV-6 Infection and Chemokine RANTES Signaling Pathway Disturbance in Patients with Autoimmune Thyroiditis. Viruses 12:N/A (2020). PubMed: 32604892
- Cai CC et al. Glycine Protects against Hypoxic-Ischemic Brain Injury by Regulating Mitochondria-Mediated Autophagy via the AMPK Pathway. Oxid Med Cell Longev 2019:4248529 (2019). PubMed: 30881590
- Huang S et al. BMP2 Is Related to Hirschsprung's Disease and Required for Enteric Nervous System Development. Front Cell Neurosci 13:523 (2019). PubMed: 31849612
- Saxena S et al. ATR Signaling Uncouples the Role of RAD51 Paralogs in Homologous Recombination and Replication Stress Response. Cell Rep 29:551-559.e4 (2019). PubMed: 31618626
- Morais T et al. GLP-1 induces alpha cell proliferation and overrides leptin suppression induced by negative energy balance in vagotomized rats. J Cell Biochem N/A:N/A (2019). PubMed: 30994953
- Saxena S et al. XRCC2 Regulates Replication Fork Progression during dNTP Alterations. Cell Rep 25:3273-3282.e6 (2018). PubMed: 30566856
- Chen H et al. Identification of the potential molecular mechanism and driving mutations in the pathogenesis of familial intestinal gastric cancer by whole exome sequencing. Oncol Rep 40:2316-2324 (2018). PubMed: 30106433