Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6729)
Key features and details
- Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WB
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Mouse -
Tested applications
Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details -
Immunogen
Mouse IgG whole molecule
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Diluted stock solutions should be used immediately and not be frozen. Do Not Freeze. Please see notes section. Store undiluted. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 1% BSA, 0.79% Tris HCl, 0.01% Magnesium chloride, 0.001% Zinc chloride, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons) -
Clonality
Polyclonal -
General notes
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Dot blot |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent dilution.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Immunomicroscopy |
Use at an assay dependent concentration.
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ICC/IF |
1/500 - 1/2000.
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WB |
Use at an assay dependent dilution.
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Notes |
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Dot blot
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent dilution. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Immunomicroscopy
Use at an assay dependent concentration. |
ICC/IF
1/500 - 1/2000. |
WB
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab6729 has been referenced in 24 publications.
- Zegeye ED et al. Vaccine Candidate Double Mutant Variants of Enterotoxigenic Escherichia coli Heat-Stable Toxin. Vaccines (Basel) 10:N/A (2022). PubMed: 35214698
- Wu P et al. Cyclic growth of dermal papilla and regeneration of follicular mesenchymal components during feather cycling. Development 148:N/A (2021). PubMed: 34344024
- Kuznetsov JN et al. BAP1 regulates epigenetic switch from pluripotency to differentiation in developmental lineages giving rise to BAP1-mutant cancers. Sci Adv 5:eaax1738 (2019). PubMed: 31555735
- Shan X et al. Edoxaban improves atrial fibrillation and thromboembolism through regulation of the Wnt-ß-induced PI3K/ATK-activated protein C system. Exp Ther Med 17:3509-3517 (2019). PubMed: 30988731
- Govasli ML et al. Virus-like particle-display of the enterotoxigenic Escherichia coli heat-stable toxoid STh-A14T elicits neutralizing antibodies in mice. Vaccine 37:6405-6414 (2019). PubMed: 31515145