Rabbit Anti-Mouse IgG H&L (Biotin) (ab6727)
Key features and details
- Rabbit Anti-Mouse IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WB
Overview
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Product name
Rabbit Anti-Mouse IgG H&L (Biotin)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Mouse -
Tested applications
Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC/IF, WBmore details -
Immunogen
Mouse IgG whole molecule
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Dot blot | ||
ELISA | ||
IHC-P | ||
IHC-Fr | ||
Immunomicroscopy | ||
ICC/IF | ||
WB |
Notes |
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Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab6727 has been referenced in 2 publications.
- Binch A et al. Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues. JOR Spine 3:e1098 (2020). PubMed: 33015573
- Han T et al. HIF-1a contributes to tube malformation of human lymphatic endothelial cells by upregulating VEGFR-3. Int J Oncol 54:139-151 (2019). PubMed: 30431105