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Rabbit Anti-Mouse IgG H&L (Biotin) (ab97044)

Submit a review Q&A (3)References (5)

Key features and details

  • Rabbit Anti-Mouse IgG H&L (Biotin)
  • Conjugation: Biotin
  • Host species: Rabbit
  • Isotype: IgG
  • Suitable for: ELISA, ICC/IF, IHC-P, WB

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase FITC HRP Texas Red ® TRITC Unconjugated Unconjugated

Overview

  • Product name

    Rabbit Anti-Mouse IgG H&L (Biotin)
    See all IgG secondary antibodies

  • Host species

    Rabbit

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

  • Tested applications

    Suitable for: ELISA, ICC/IF, IHC-P, WBmore details

  • Conjugation

    Biotin

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.

  • Conjugation notes

    Biotin/ antibody protein ratio is 4:1

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/20000 - 1/400000. (Primary).
ICC/IF
1/200 - 1/1000.
IHC-P
1/500 - 1/5000.
WB
1/20000 - 1/400000. (Chemiluminescent).
Notes
ELISA
1/20000 - 1/400000. (Primary).
ICC/IF
1/200 - 1/1000.
IHC-P
1/500 - 1/5000.
WB
1/20000 - 1/400000. (Chemiluminescent).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (5)

Publishing research using ab97044? Please let us know so that we can cite the reference in this datasheet.

ab97044 has been referenced in 5 publications.

  • Pérez-Santos I  et al. Distribution of the Noradrenaline Innervation and Adrenoceptors in the Macaque Monkey Thalamus. Cereb Cortex 31:4115-4139 (2021). PubMed: 34003210
  • Pérez-Castrillo S  et al. Effect of mesenchymal stem cells combined with chondroitin sulfate in an in vitro model of osteoarthritis. Am J Transl Res 13:5928-5942 (2021). PubMed: 34306335
  • Qian W  et al. High expression of carcinoembryonic antigen-associated cell adhesion molecule 1 is associated with microangiogenesis in esophageal squamous cell carcinoma. Transl Cancer Res 9:4762-4769 (2020). PubMed: 35117839
  • Zhou M  et al. Spontaneous hypothermia ameliorated inflammation and neurologic deficit in rat cardiac arrest models following resuscitation. Mol Med Rep 17:2127-2136 (2018). PubMed: 29207113
  • Cottle DL  et al. c-MYC-induced sebaceous gland differentiation is controlled by an androgen receptor/p53 axis. Cell Rep 3:427-41 (2013). PubMed: 23403291

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

I have got a question about second antibodies used for Western Blot. At the moment I am using Polyclonal Rabbit-anti-mouse immunoglobulins/HRP as second antibody. We would like to add a step for strengthening the signal, like using Biotin. Is it possible to use cat# ab97044 as second antibody in combination with cat# ab6651 for the detection with DAB? Thank you for your answer.

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Answer

Thank you for contacting us.

While it is theoretically possible to amplify your signal by using a biotinylated secondary antibody with an anti-biotin tertiary antibody, it is not commonly done. Because streptavidin binds to biotin with a very high affinity, it is very commonly used to provide amplification in biotin systems in IHC or ELISA. For example, you could use streptavidin-HRP (ab64269) to detect the biotinylated secondary antibody. When using biotinylated secondary antibodies in WB, it is important to block with BSA instead of milk. Milk contains endogenous biotins that can cause very high background.

To increase the WB signal, it is more common to use a more sensitive ECL reagent or a longer exposure. Alternatively, you could try things like enriching your samples by subcellular fractionation(ab109719), loading more protein, using more primary antibody, or incubating overnight in the primary antibody. Some researchers also produce a slightly amplified signal by using a tertiary antibody. For example, you could use an unconjugated rabbit anti-mouse (ab97042)followed by an HRP conjugated anti-rabbit (ab97051).

I hope this helps, please let me know if you need any additional information or assistance.

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Question

Hello, I just wanted to confirm that this antibody has the ability to recognize both "mouse and rabbit" regions and can be used as secondary antibody for recognizing Mouse Antibodies and Rabbit Antibodies. Also is there any technical specifications as compare to individual secondary antibodies ab64256 (Anti-rabbit) and ab97044 (Anti-mouse)? Let me know as soon as possible. Thanks and Warm Regards,

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Answer

Thank you for your inquiry. ab64257 is indeed suitable for the detection of Mouse and Rabbit IgG. It can be used as a secondary antibody when used with rabbit or mouse primary antibodies. About ab64256 and ab97044, all of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention. Please do not hesitate to contact us again if you need further assistance.

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Question

Customer kindly requested Biotin conjugated secondary antibodies for ab2378, ab5690, ab103573, ab10558, ab955, ab53444, ab9018

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Answer

Thank you for your interest in Abcam products. ab2378, ab955 and ab9018 are mouse monoclonal antibodies. I would recommend using ab97044 as a secondary antibody; https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Biotin-ab97044.html ab5690, ab103573, ab10558 are rabbit polyclonal antibodies. I would recommend using ab6720; https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-ab6720.html ab53444 is a rat monoclonal antibody. I would recommend using ab6733; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6733.html To let you know we are running a promotion '4 for 3' on primaries; if you could select 1 more primary antibody then you will be entitled to 8 primary antibodies for the price of 6. Use the discount code and follow the instructions given on website. I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

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