Rabbit Anti-Mouse IgG H&L (Biotin) (ab97044)
Key features and details
- Rabbit Anti-Mouse IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ELISA, ICC/IF, IHC-P, WB
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Mouse IgG H&L (Biotin)
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
Tested applications
Suitable for: ELISA, ICC/IF, IHC-P, WBmore details -
Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells. -
Conjugation notes
Biotin/ antibody protein ratio is 4:1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/20000 - 1/400000. (Primary).
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ICC/IF |
1/200 - 1/1000.
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IHC-P |
1/500 - 1/5000.
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WB |
1/20000 - 1/400000. (Chemiluminescent).
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Notes |
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ELISA
1/20000 - 1/400000. (Primary). |
ICC/IF
1/200 - 1/1000. |
IHC-P
1/500 - 1/5000. |
WB
1/20000 - 1/400000. (Chemiluminescent). |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab97044 has been referenced in 5 publications.
- Pérez-Santos I et al. Distribution of the Noradrenaline Innervation and Adrenoceptors in the Macaque Monkey Thalamus. Cereb Cortex 31:4115-4139 (2021). PubMed: 34003210
- Pérez-Castrillo S et al. Effect of mesenchymal stem cells combined with chondroitin sulfate in an in vitro model of osteoarthritis. Am J Transl Res 13:5928-5942 (2021). PubMed: 34306335
- Qian W et al. High expression of carcinoembryonic antigen-associated cell adhesion molecule 1 is associated with microangiogenesis in esophageal squamous cell carcinoma. Transl Cancer Res 9:4762-4769 (2020). PubMed: 35117839
- Zhou M et al. Spontaneous hypothermia ameliorated inflammation and neurologic deficit in rat cardiac arrest models following resuscitation. Mol Med Rep 17:2127-2136 (2018). PubMed: 29207113
- Cottle DL et al. c-MYC-induced sebaceous gland differentiation is controlled by an androgen receptor/p53 axis. Cell Rep 3:427-41 (2013). PubMed: 23403291