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Rabbit Anti-Mouse IgG H&L (HRP) preadsorbed (ab7075)

Submit a review Q&A (3)References (4)

Key features and details

  • Rabbit Anti-Mouse IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Rabbit
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

Overview

  • Product name

    Rabbit Anti-Mouse IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Rabbit

  • Target species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

  • Minimal
    cross-reactivity


    Human more details

  • Immunogen

    Full length native Mouse IgG (purified).

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.

  • Conjugation notes

    Horseradish Peroxidase (HRP)

  • Clonality

    Polyclonal

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/200 - 1/1000.
Dot blot
Use at an assay dependent dilution.
ELISA
1/5000 - 1/20000.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
WB
1/500 - 1/2500.
Notes
ICC/IF
1/200 - 1/1000.
Dot blot
Use at an assay dependent dilution.
ELISA
1/5000 - 1/20000.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
WB
1/500 - 1/2500.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (4)

Publishing research using ab7075? Please let us know so that we can cite the reference in this datasheet.

ab7075 has been referenced in 4 publications.

  • Khodja Y & Samuels ME Ethanol-mediated upregulation of APOA1 gene expression in HepG2 cells is independent of de novo lipid biosynthesis. Lipids Health Dis 19:144 (2020). PubMed: 32563265
  • Sun Y  et al. Factor H Is Bound by Outer Membrane-Displayed Carbohydrate Metabolism Enzymes of Extraintestinal Pathogenic Escherichia coli and Contributes to Opsonophagocytosis Resistance in Bacteria. Front Cell Infect Microbiol 10:592906 (2020). PubMed: 33569353
  • Whelan DR  et al. Spatiotemporal dynamics of homologous recombination repair at single collapsed replication forks. Nat Commun 9:3882 (2018). PubMed: 30250272
  • Gupta V  et al. Inhaled PLGA particles of prostaglandin E1 ameliorate symptoms and progression of pulmonary hypertension at a reduced dosing frequency. Mol Pharm 10:1655-67 (2013). WB . PubMed: 23485062

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Question

Dear Sir or Madam,

We are a biophysical lab and we are working with recombinantly expressed HBV proteins. To validate some of our recombinant protein we're now looking into co-immunoprecipitation and western blotting. These techniques have never been used in our lab, so I have a few questions. To establish antibody techniques in our lab I am interested in HBx antibodies, but once protocols have been established we'd be interested in antibodies against other HBV proteins as well.

Could you send me a quote for the antibodies listed below?


Anti-6X His tag® antibody [3H2201] (ab15149)

Anti-6X His tag® antibody (ab125262)

Anti-6X His tag® antibody (Agarose) (ab90663)

Anti-Hepatitis B Virus X antigen antibody [3F6-G10] (ab235)
Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741)
Anti-Hepatitis B Virus X antigen antibody (ab39716)

Anti-p53 antibody [PAb 240] (ab26)

I'm also interested in secondary antibodies for the use in western blots. Could you recommend a matching secondary anti-mouse antibody for WB use?
WouldRabbit anti-Mouse IgG H&L (HRP) secondary antibody (ab7075) be a good choice?

Reading the posts in your support section I can see that the epitopes of the HBx antibodies are not known. Has anything changed since then?
And my last question would be if you supply any conformation specific antibodies against HBV core protein (HBc).

Best wishes

Read More
Answer

Sorry for the delay in getting back to you. I now have further information to share with you.

I have checked into whether anything is know about the epitope of the anti-Hepatitis B Virus X antigen antibodies ab235, ab2741 and ab39716. I can only provide further information for ab235 additionally to the information provided on the datasheet of the antibodies. The epitope recognised by ab235 is thought to recognise an epitope of the following sequence:

DVLCLRPVGASESR.

I was incorrect in my last email to say that ab235 had not been tested in Western blotting. It have been tested but we have had mixed reports from customers so we would not recommend buying this antibody with that application in mind.

I have also looked into the anti-Hepatitis B Virus Core Antigen antibodies we have and if anything is known of the conformation of the epitope is known. Unfortunately, I could not find any antibodies where this information had been established and most are able to recognise both the denatured and native form of the protein.

I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

Read More

Question

Dear Sir or Madam,

We are a biophysical lab and we are working with recombinantly expressed HBV proteins. To validate some of our recombinant protein we're now looking into co-immunoprecipitation and western blotting. These techniques have never been used in our lab, so I have a few questions. To establish antibody techniques in our lab I am interested in HBx antibodies, but once protocols have been established we'd be interested in antibodies against other HBV proteins as well.

Could you send me a quote for the antibodies listed below?


Anti-6X His tag® antibody [3H2201] (ab15149)

Anti-6X His tag® antibody (ab125262)

Anti-6X His tag® antibody (Agarose) (ab90663)

Anti-Hepatitis B Virus X antigen antibody [3F6-G10] (ab235)
Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741)
Anti-Hepatitis B Virus X antigen antibody (ab39716)

Anti-p53 antibody [PAb 240] (ab26)

I'm also interested in secondary antibodies for the use in western blots. Could you recommend a matching secondary anti-mouse antibody for WB use?
WouldRabbit anti-Mouse IgG H&L (HRP) secondary antibody (ab7075) be a good choice?

Reading the posts in your support section I can see that the epitopes of the HBx antibodies are not known. Has anything changed since then?
And my last question would be if you supply any conformation specific antibodies against HBV core protein (HBc).

Best wishes

Read More
Answer

Thank you for contacting us and your interest in our products.

The request for a quote for the antibodies mentioned has been passed on to one of my colleagues who will get back to you shortly with this information. In regards to your other questions:

The Rabbit anti-Mouse IgG H&L (HRP) secondary antibody (ab7075) should work well with ab15149, ab2741, ab235 and ab26. You would need a different secondary antibody for all the other antibodies mentioned as they are raised in rabbit. You may wantto consider the Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080). Please bear in mind that we have as yet not tested the anti-Hepatitis B Virus X antigen antibody [3F6-G10] (ab235) in Western blotting or immunoprecipitation. This does not mean that it would not work, simply that we have not tested it ourselves as yet and would therefore not he able to guarantee its performance in these applications.

We have some good resources in setting up both immunoprecipitation and Western blotting experiments from the following link:

https://docs.abcam.com/pdf/misc/abcam-protocols-book-2010.pdf

Some further troubleshooting tips can also be found here:

https://www.abcam.com/index.html?pageconfig=resource&rid=11382

If you would like any help with this please do let us know.

I will check if any further information is know about the epitopes recognised by the anti-HBx antibodies mentioned and will get back to you with this information as soon as I have it. I will also look into your final question ifwe have any conformation specific antibodies against HBV core protein (HBc).

As you will be setting up these experiments from scratch, you may be interested in looking at our Western blotting range:

https://www.abcam.com/index.html?pageconfig=resource&rid=14709&source=pagetrap&viapagetrap=optiblot

The link above will take you not only to our Optiblot range of gels which provide superior durability and ease of use compared to many standard gels, but also the PVDF and nitrocellulose membranes and Ecl Detection kits we have available.

If you would like any further information on these products please do let us know.

Read More

Question

Inquiry: Dear, Does this product cross reacts with IgG from other species (e.g. human, rat, ...)? If so do you have another one that does react with mouse IgG but not with human IgG?

Read More
Answer

Thank you for contacting us.
As this antibody has only been purified against it immunogen, it may cross- react with other species.
I have picked out three pre-adsorbed secondary antibodies, which show minimal cross-reactivity to human IgG:
https://www.abcam.com/Rabbit-Mouse-IgG-HL-HRP-preadsorbed-ab7075.html https://www.abcam.com/Rabbit-Mouse-IgG-HL-HRP-preadsorbed-ab7075.html.
https://www.abcam.com/Rabbit-Fab2-Mouse-IgG-HL-HRP-pre-adsorbed-ab98780.html https://www.abcam.com/Rabbit-Fab2-Mouse-IgG-HL-HRP-pre-adsorbed-ab98780.html.
https://www.abcam.com/Goat-Mouse-IgG-HL-HRP-preadsorbed-ab98808.html https://www.abcam.com/Goat-Mouse-IgG-HL-HRP-preadsorbed-ab98808.html.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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