Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
Key features and details
- Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Rabbit
- Suitable for: ICC/IF, IHC-P
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC)
See all IgG + IgM + IgA secondary antibodies -
Host species
Rabbit -
Target species
Mouse -
Specificity
Antibodies were raised in rabbit to mouse IgG, IgM and IgA -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.50
Constituents: 0.164% Sodium phosphate, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Specific antibodies were absorpted by incubation with Sepharose-bound mouse immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized mouse immunoglobulins a minimum of 65% protein bound. -
Conjugation notes
The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate. -
Clonality
Polyclonal -
General notes
F/P ratio is 3-5 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence of normal tissue in contrast to specific staining of sections treated with monoclonal mouse anti-human collagen antibodies -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent dilution. PubMed: 15483223
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| IHC-P |
Use at an assay dependent dilution.
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| Notes |
|---|
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ICC/IF
Use at an assay dependent dilution. PubMed: 15483223 |
|
IHC-P
Use at an assay dependent dilution. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab8517 has been referenced in 11 publications.
- Zhao M et al. The role and potential mechanism of p75NTR in mineralization via in vivo p75NTR knockout mice and in vitro ectomesenchymal stem cells. Cell Prolif 53:e12758 (2020). PubMed: 31922317
- Akyash F et al. Derivation of new human embryonic stem cell lines (Yazd1-3) and their vitrification using Cryotech and Cryowin tools: A lab resources report. Int J Reprod Biomed (Yazd) 17:891-906 (2019). PubMed: 31970311
- Kolodziej M et al. First molecular characterization of Dobrava-Belgrade virus found in Apodemus flavicollis in Poland. Ann Agric Environ Med 25:368-373 (2018). PubMed: 29936795
- Omidi A et al. Prenatal transplantation of epidermal neural crest stem cells in malformation of cortical development mouse model. Microsc Res Tech 80:394-405 (2017). PubMed: 28001323
- Ahn J et al. Transplantation of human Wharton's jelly-derived mesenchymal stem cells highly expressing TGFß receptors in a rabbit model of disc degeneration. Stem Cell Res Ther 6:190 (2015). IHC ; Human . PubMed: 26432097