Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 647) (ab172327)
![Flow Cytometry - Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 647) (ab172327)](https://www.abcam.com/ps/products/172/ab172327/Images/ab172327-226308-rat-monoclonal-sb74g-anti-mouse-igg2b-gamma-chain-alexa-fluor-647-flow-cytometry.jpg "Flow Cytometry - Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 647) (ab172327)")
Key features and details
- Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 647)
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Host species: Rat
- Isotype: IgG2b
- Suitable for: IHC-P, ELISA, ICC/IF, Flow Cyt
Overview
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Product name
Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 647)
See all IgG2b secondary antibodies -
Host species
Rat -
Target species
Mouse -
Specificity
Reacts with the y2b heavy chain of mouse IgG2b. Minimal reaction with other mouse immunoglobulins as determined by ELISA. May react with immunoglobulins from other species. -
Tested applications
Suitable for: IHC-P, ELISA, ICC/IF, Flow Cytmore details -
Immunogen
The details of the immunogen for this antibody are not available.
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Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
SB74g -
Isotype
IgG2b -
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Associated products
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Alternative Versions
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
1/200 - 1/1000.
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| Flow Cyt |
1/1000.
ab176105 - Mouse monoclonal IgG2b (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody. |
| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
1/200 - 1/1000. |
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Flow Cyt
1/1000. ab176105 - Mouse monoclonal IgG2b (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody. |
Images
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Flow Cytometry - Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 647) (ab172327)
Overlay histogram showing subclass specificity of ab172327. HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% serum / 0.3M glycine to block non-specific protein-protein interactions followed by the primary antibody (1μg/1x106 cells) as indicated: ab8982 (Mouse IgG1), ab49105 (Mouse IgG2a), ab16965 (Mouse IgG2b), ab60602 (Mouse IgG3), ab55602 (Mouse IgM) for 30 min at 22ºC. The secondary antibody (ab172327 - Rat monoclonal (SB74g) Anti-Mouse IgG2b, gamma chain - Alexa Fluor® 647) was used at 1/1000 dilution for 30 min at 22ºC. Unlabelled (blue line) and secondary only (black line) samples were also used as negative controls. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635nm) and 675/30 bandpass filter.
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![Alexa Fluor® - Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 647) (ab172327)](https://www.abcam.com/ps/products/172/ab172327/Images/ab172327-210592-rat-monoclonal-sb74g-anti-mouse-igg2b-gamma-chain-alexa-fluor-647-alexa-fluor.jpg)
Alexa Fluor® - Rat monoclonal [SB74g] Anti-Mouse IgG2b gamma chain (Alexa Fluor® 647) (ab172327)Emission spectra of Alexa Fluor® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab172327 has been referenced in 3 publications.
- Jeffries MA et al. mTOR Signaling Regulates Metabolic Function in Oligodendrocyte Precursor Cells and Promotes Efficient Brain Remyelination in the Cuprizone Model. J Neurosci 41:8321-8337 (2021). PubMed: 34417330
- Nomani A et al. Evaluation of genotoxicity and mutagenic effects of vector/DNA nanocomplexes in transfected mesenchymal stem cells by flow cytometry. Acta Biomater 74:236-246 (2018). PubMed: 29783088
- McLane LE et al. Loss of Tuberous Sclerosis Complex1 in Adult Oligodendrocyte Progenitor Cells Enhances Axon Remyelination and Increases Myelin Thickness after a Focal Demyelination. J Neurosci 37:7534-7546 (2017). PubMed: 28694334