Rat monoclonal [SB77e] Anti-Mouse IgG1 (Biotin), pre-adsorbed (ab99604)
![Flow Cytometry - Rat monoclonal [SB77e] Anti-Mouse IgG1 (Biotin), pre-adsorbed (ab99604)](https://www.abcam.com/ps/products/99/ab99604/Images/ab99604-113736-rat-monoclonal-sb77e-anti-mouse-igg1-biotin-pre-adsorbed-flow-cytometry.jpg "Flow Cytometry - Rat monoclonal [SB77e] Anti-Mouse IgG1 (Biotin), pre-adsorbed (ab99604)")
Key features and details
- Rat monoclonal [SB77e] Anti-Mouse IgG1 (Biotin), pre-adsorbed
- Conjugation: Biotin
- Host species: Rat
- Isotype: IgG2b
- Suitable for: ELISA, Flow Cyt
Related conjugates and formulations
Overview
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Product name
Rat monoclonal [SB77e] Anti-Mouse IgG1 (Biotin), pre-adsorbed
See all IgG1 secondary antibodies -
Host species
Rat -
Target species
Mouse -
Specificity
ab99604 reacts with Mouse IgG1 as demonstrated by ELISA. Minimal reaction to Human, Goat and Rabbit IgG. Each batch of Rat monoclonal [SB77e] Anti-Mouse IgG1 (Biotin), pre-adsorbed is tested to show minimal cross reactivity with IgM, IgA, and other subsets of IgG.
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Tested applications
Suitable for: ELISA, Flow Cytmore details -
Minimal
cross-reactivity
Goat, Human, Rabbit more details -
Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SB77e -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
- Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (FITC) preadsorbed (ab133859)
- Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L preadsorbed (ab99601)
- Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (Alkaline Phosphatase) preadsorbed (ab99602)
- Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (HRP) preadsorbed (ab99603)
- Rat monoclonal [SB77e] Anti-Mouse IgG1 H&L (PE) preadsorbed (ab99605)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
1/5000 - 1/20000.
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| Flow Cyt |
Use at an assay dependent dilution.
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| Notes |
|---|
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ELISA
1/5000 - 1/20000. |
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Flow Cyt
Use at an assay dependent dilution. |
Images
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Flow Cytometry - Rat monoclonal [SB77e] Anti-Mouse IgG1 (Biotin), pre-adsorbed (ab99604)Top: (Negative control) ab99604 staining for Mouse IgG1 in Human blood at 1µg/106 cells.
Bottom: ab99604 staining in Human blood incubated with mouse anti-Human CD3 (IgG1 isotype) and Streptavidin-FITC.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab99604 has been referenced in 1 publication.
- Park MH et al. Development of a high-yield technique to isolate spermatogonial stem cells from porcine testes. J Assist Reprod Genet 31:983-91 (2014). PubMed: 24938360