HeLa nuclear extract lysate (ab150036)
Overview
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Product name
HeLa nuclear extract lysate
See all HeLa lysates -
General notes
We recommend aliquoting the extracts into single use fractions and then storing them at -80°C.This HeLa Nuclear Extract was prepared using a standard nuclear lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a non-reducing lithium dodecyl sulfate sample loading buffer (LDS).
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituent: 10% DTT
Note: This lysate was normalised with SDS-PAGE sample buffer to current concentration. -
Concentration information loading... -
Lysate notes
This HeLa Nuclear Extract was prepared using a standard nuclear lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a non-reducing lithium dodecyl sulfate sample loading buffer (LDS). -
Background
HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.
Images
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SDS-PAGE - HeLa nuclear extract lysate (ab150036)All lanes stained with Optiblot Blue (ab119211) (20 ml)
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)
Lysates/proteins at 10 µg per lane. -
Western blot - HeLa nuclear extract lysate (ab150036)All lanes : Anti-HDAC1 antibody (ab19845) at 1 µg/ml
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 :HeLa nuclear extract lysate (ab150036)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Western blot - HeLa nuclear extract lysate (ab150036)All lanes : Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (ab63766) at 1 µg/ml
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 :HeLa nuclear extract lysate (ab150036)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Observed band size: 45 kDa why is the actual band size different from the predicted? -
Western blot - HeLa nuclear extract lysate (ab150036)All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/ml
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 :HeLa nuclear extract lysate (ab150036)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Observed band size: 42 kDa why is the actual band size different from the predicted? -
Western blot - HeLa nuclear extract lysate (ab150036)All lanes : Anti-beta Tubulin antibody (ab21057) at 1 µg/ml
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 :HeLa nuclear extract lysate (ab150036)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Observed band size: 51 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab150036 has been referenced in 1 publication.
- Lee WH et al. DNA primase polypeptide 1 (PRIM1) involves in estrogen-induced breast cancer formation through activation of the G2/M cell cycle checkpoint. Int J Cancer 144:615-630 (2019). PubMed: 30097999